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J09:0130j - AT the the [Ns. J09:0140a - JJ present present . J09:0140b - NN1n work work .Ns]P:p] J09:0140c - JJ whole whole [Np:S. J09:0140d - NN2 sera serum .Np:S] J09:0140e - VH0 have have [Vfp. J09:0140f - VBN been be . J09:0140g - VVNt fractionated fractionate .Vfp] J09:0140h - IIb by by [Pb:h. J09:0140i - NN1u chromatography chromatography [Ns:103. J09:0140j - II on on [P. J09:0145a S FOc DEAE - [Ns. J09:0145b - YH + - . J09:0145c - NN1u +cellulose cellulose .Ns]P] J09:0145d - YG - - [Tg[s103.s103] J09:0150a - VVGt using use [Vg.Vg] J09:0150b - JJ single single [Np:o105. J09:0150c - NN2 gradients gradient . J09:0150d - JJ similar similar [Jh. J09:0150e - IIt to to [P. J09:0150f - DD2a those those [Dp:105. J09:0150g - YG - - [Tn[S105.S105] J09:0150h - VVNt described describe [Vn.Vn] J09:0160a - IIb by by [Pb:a. J09:0160b - NP1s Sober Sober [Nn[NP1s&. J09:0160c - CC and and [NP1s+. J09:0160d - NP1s Peterson Peterson .NP1s+]NP1s&]Nn]Pb:a]Tn]Dp:105]P]Jh]Np:o105]Tg]Ns:103]Pb:h] J09:0160e - YC +, - . J09:0160f - CC and and [S+. J09:0160g - JJ certain certain [Np:S. J09:0160h - JJ chemical chemical [JJ&. J09:0160i - CC and and [JJ+. J09:0160j - JJ serological serological .JJ+]JJ&] J09:0160k - NN2 properties property . J09:0170a - IO of of [Po. J09:0170b - AT the the [Np:107. J09:0170c - NN2 fractions fraction . J09:0170d - YG - - [Tg[s107.s107] J09:0170e - VVGt containing contain [Vg.Vg] J09:0170f - NN2 antibodies antibody [Np:o. J09:0170g - IO of of [Po. J09:0170h - AT the the [Np. J09:0170i S FOs ABO - [FOs&. J09:0170j - CC and and [FWs+. J09:0170k S FWs Rh - .FWs+]FOs&] J09:0180a - NN2 systems system .Np]Po]Np:o]Tg]Np:107]Po]Np:S] J09:0180b - VH0 have have [Vfp. J09:0180c - VBN been be . J09:0180d - VVNt described describe .Vfp]S+]S] J09:0180e - YF +. - .O] J09:0180f - YBL - [Oh. J09:0180g - NN2 MATERIALS material [Np[NN2&. J09:0180h - CC AND and [NN2+. 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J09:0200r - VVNv taken take .Vwp] J09:0210a - II from from [P:q. J09:0210b - RR recently recently [Np[Tn[R:t.R:t] J09:0210c - VVNt sensitized sensitize [Vn.Vn]Tn] J09:0210d - NN2 individuals individual .Np]P:q]S] J09:0210e - YF +. - . J09:0210f - MC1 One one [S[Ms:s.Ms:s] J09:0210g - VVDt contained contain [Vd.Vd] J09:0210h - JJ complete complete [Ns:o. J09:0210i - NN1c antibody antibody .Ns:o] J09:0220a - CC and and [S+. J09:0220b - VHD had have [Vd.Vd] J09:0220c - AT1 a a [Ns:o. J09:0220d - NN1n titer titre . J09:0220e - IO of of [Po. J09:0220f - MC1n 1 - [Ms. J09:0220g - IIx +: - [P. J09:0220h - MCn +512 - .P]Ms]Po]Ns:o] J09:0220i - II in in [P:p. J09:0220j - NN1u saline saline .P:p]S+]S] J09:0220k - YF +. - . J09:0220m - AT The the [S[Nj:s. J09:0220n - MDo second second .Nj:s] J09:0220p - VVDt contained contain [Vd.Vd] J09:0220q - JJ incomplete incomplete [Ns:o. J09:0230a - NN1c antibody antibody .Ns:o] J09:0230b - CC and and [S+. J09:0230c - VVDv showed show [Vd.Vd] J09:0230d - NN2 titers titre [Np:o. J09:0230e - IO of of [Po. J09:0230f - MC1n 1 - [Ms. J09:0230g - IIx +: - [P. J09:0230h - MCn +256 - .P]Ms]Po]Np:o] J09:0230i - II in in [P:p. J09:0230j - NN1u albumin albumin .P:p] J09:0230k - CC and and [S+. J09:0230m - MC1n 1 - [Ms:o. J09:0230n - IIx +: - [P. J09:0230p - MCn +2048 - .P]Ms:o] J09:0230q - IIb by by [Pb:h. J09:0240a - AT the the [Ns. J09:0240b - JJ indirect indirect . J09:0240c - NP1s Coombs Coombs [Nns.Nns] J09:0240d - NN1c test test .Ns]Pb:h]S+]S+]S] J09:0240e - YF +. - . J09:0240f - AT The the [S[Nj:s111. J09:0240g - MD third third . J09:0240h - YC +, - . J09:0240i - YG - - [Tg[s111.s111] J09:0240j - VVGt containing contain [Vg.Vg] J09:0240k - AT the the [Ns:o. J09:0240m - JJ mixed mixed . J09:0240n - NN1n type type . J09:0240p - IO of of [Po. J09:0240q - JJ complete complete [Np[JJ&. J09:0250a - CC and and [JJ+. J09:0250b - JJ incomplete incomplete .JJ+]JJ&] J09:0250c - NN2 antibodies antibody .Np]Po]Ns:o]Tg] J09:0250d - YC +, - .Nj:s111] J09:0250e - VHD had have [Vd.Vd] J09:0250f - NN2 titers titre [N:o. J09:0250g - IO of of [Po. J09:0250h - MC1n 1 - [Ms. J09:0250i - IIx +: - [P. J09:0250j - MCn +256 - .P]Ms]Po] J09:0250k - II in in [P. J09:0250m - NN1u saline saline .P] J09:0250n - YC +, - . J09:0250p - MC1n 1 - [N-[Ms. J09:0250q - IIx +: - [P. J09:0250r - MCn +512 - .P]Ms] J09:0260a - II in in [P. J09:0260b - NN1u albumin albumin .P]N-] J09:0260c - CC and and [N+. J09:0260d - MC1n 1 - [Ms. J09:0260e - IIx +: - [P. J09:0260f - MCn +1024 - .P]Ms] J09:0260g - IIb by by [Pb. J09:0260h - AT the the [Ns. J09:0260i - JJ indirect indirect . J09:0260j - NP1s Coombs Coombs [Nns.Nns] J09:0260k - NN1c test test .Ns]Pb]N+]N:o]S] J09:0260m - YF +. - . J09:0260n - II In in [S[P:m. J09:0260p - NN1n addition addition .P:m] J09:0270a - MC1 one one [Ns:S. J09:0270b - NN1u serum serum .Ns:S] J09:0270c - VBDZ was be [Vsp. J09:0270d - VVNv obtained obtain .Vsp] J09:0270e - II from from [P:q. J09:0270f - AT1 a a [Ns:113. J09:0270g - NN1c donor donor . J09:0270h - YPL ( - . J09:0270i A NP1i +R. - [Nns@. J09:0270j A NP1i E. - .Nns@] J09:0270k - YPR +) - . J09:0270m - PNQSr who who [Fr[Nq:S113.Nq:S113] J09:0270n - VHD had have [Vdfp. J09:0270p - VBN been be . J09:0270q - VVNt sensitized sensitize .Vdfp] J09:0280a - MCn 6 - [Rx:t[Np. J09:0280b - NNT2 years year .Np] J09:0280c - RR previously previously .Rx:t]Fr]Ns:113]P:q]S] J09:0280d - YF +. - . J09:0280e - DD1i This this [S[Ns:s. J09:0280f - NN1u serum serum .Ns:s] J09:0280g - VVDt exhibited exhibit [Vd.Vd] J09:0280h - NN2 titers titre [N:o. J09:0280i - IO of of [Po. J09:0280j - MC1n 1 - [Ms. J09:0280k - IIx +: - [P. J09:0280m - MCn +16 - .P]Ms]Po] J09:0280n - II in in [P. J09:0280p - NN1u albumin albumin .P] J09:0290a - CC and and [N+. J09:0290b - MC1n 1 - [Ms. J09:0290c - IIx +: - [P. J09:0290d - MCn +256 - .P]Ms] J09:0290e - IIb by by [Pb. J09:0290f - AT the the [Ns. J09:0290g - JJ indirect indirect . J09:0290h - NP1s Coombs Coombs [Nns.Nns] J09:0290i - NN1c test test .Ns]Pb]N+]N:o]S] J09:0290j - YF +. - . J09:0290k - DD2i These these [S[Np:S. J09:0290m - NN1c antibody antibody . J09:0290n - NN2 titers titre .Np:S] J09:0300a - VBDR were be [Vwp. J09:0300b - VVNv determined determine .Vwp] J09:0300c - IIb by by [Pb:a. J09:0300d - NN1n reaction reaction [Ns. J09:0300e - IW with with [P. J09:0300f - JJ homozygous homozygous [Np. J09:0300g - FO - . J09:0300h - JJ red red . J09:0300i - NN2 cells cell .Np]P]Ns]Pb:a]S] J09:0300j - YF +. - .O] J09:0300k - YBL - [Oh. J09:0300m - JJ SEROLOGICAL serological [Ns. J09:0310a - NN1n TECHNIQUE technique .Ns] J09:0310b - YF +. - . J09:0310c - YBR - .Oh] J09:0310d - FB Anti anti [O[S[Np:S[J. J09:0310e - YH + - . J09:0310f S ZZ1 +A - . J09:0310g - CC and and [J+. J09:0310h - FB anti anti . J09:0310i - YH + - . J09:0310j S ZZ1 +B - .J+]J] J09:0310k - NN2 activities activity .Np:S] J09:0310m - VBDR were be [Vwp. J09:0310n - VVNv determined determine .Vwp] J09:0320a - II in in [P:p. J09:0320b - NN2 fractions fraction [Np. J09:0320c - II from from [P. J09:0320d - AT the the [Np. J09:0320e - NN2 sera serum . J09:0320f - IO of of [Po. J09:0320g - NNJ1c group group [Np[Nn. J09:0320h S ZZ1 A - . J09:0320i - YC +, - . J09:0320j - NNJ1c group group [Nns-. J09:0320k S ZZ1 B - .Nns-] J09:0320m - CCr or or [Nns+. J09:0320n - NNJ1c group group . J09:0320p S ZZ1 O - .Nns+]Nn] J09:0330a - NN2 donors donor .Np]Po]Np]P]Np]P:p] J09:0330b - IIb by by [Pb:h. J09:0330c - AT the the [Np. J09:0330d - JJ following following . J09:0330e - NN1n tube tube . J09:0330f - NN1n agglutination agglutination . J09:0330g - NN2 methods method .Np]Pb:h]S] J09:0330h - YF +. - . J09:0330i - MC1 One one [S[Ns:S. J09:0330j - NN1c drop drop . J09:0330k - IO of of [Po. J09:0330m - DD1q each each [Ns. J09:0340a - NN1c sample sample .Ns]Po]Ns:S] J09:0340b - VBDZ was be [Vsp. J09:0340c - VVNv added add .Vsp] J09:0340d - IIt to to [P:q. J09:0340e - MC1 one one [Ns. J09:0340f - NN1c drop drop . J09:0340g - IO of of [Po. J09:0340h - AT1 a a [Ns. J09:0340i - MCn 2 - [Nu. J09:0340j - NNUp +% - .Nu] J09:0340k - NN1u suspension suspension . J09:0340m - IO of of [Po. J09:0340n - NNJ1c group group [Np[Nn. J09:0340p - FO - . J09:0340q - CCr or or [Nns+. J09:0350a - NNJ1c group group . J09:0350b S ZZ1 B - .Nns+]Nn] J09:0350c - JJ red red . J09:0350d - NN2 cells cell .Np]Po]Ns]Po]Ns]P:q] J09:0350e - II in in [P:p. J09:0350f - AT1 a a [Ns. J09:0350g - JJ small small . J09:0350h - FO - . J09:0350i - NN1c test test . J09:0350j - NN1n tube tube .Ns]P:p]S] J09:0350k - YF +. - . J09:0350m - II In in [S[P:t. J09:0350n - DA2q several several [Np. J09:0350p - NN2 instances instance .Np]P:t] J09:0360a - NNJ1c group group [Np:S[Nns. J09:0360b S ZZ1 O - .Nns] J09:0360c - NN2 cells cell .Np:S] J09:0360d - YG - - [m115.m115] J09:0360e - VBDR were be [Vwp. J09:0360f - RR also also [R:G115.R:G115] J09:0360g - VVNt used use .Vwp] J09:0360h - IIa as as [P:e. J09:0360i - NN2 controls control .P:e]S] J09:0360j - YF +. - . J09:0360k - AT The the [S[Np:S. J09:0360m - JJ red red . J09:0360n - NN2 cells cell .Np:S] J09:0360p - VBDR were be [Vwp. J09:0360q - VVNt used use .Vwp] J09:0370a - II within within [P:t. J09:0370b - MCn 2 - [Np. J09:0370c - NNT2 days day . J09:0370d - ICSt after after [P. J09:0370e - NN1n donation donation .P]Np]P:t] J09:0370f - CC and and [S+. J09:0370g - VBDR were be [Vwp. J09:0370h - VVNv washed wash .Vwp] J09:0370i - IW with with [P:h. J09:0370j - JJ large large [Np. J09:0370k - NN2 amounts amount . J09:0370m - IO of of [Po. J09:0370n - NN1u saline saline .Po]Np]P:h] J09:0380a - ICSt before before [P:t. J09:0380b - NN1n use use .P:t]S+]S] J09:0380c - YF +. - . J09:0380d - AT The the [S[Np:S117. J09:0380e - NN2 mixtures mixture . J09:0380f - IO of of [Po. J09:0380g - NN1c sample sample [N. J09:0380h - CC plus plus [Ns+. J09:0380i - NN1c cell cell . J09:0380j - NN1u suspension suspension .Ns+]N]Po]Np:S117] J09:0380k - VBDR were be [Vwp. J09:0380m - VVNv allowed allow .Vwp] J09:0380n - YG - - [Ti:o[s117.s117] J09:0390a - TO to to [Vi. J09:0390b - VV0v stand stand .Vi] J09:0390c - II at at [P:p. J09:0390d - NN1n room room [Ns. J09:0390e - NN1n temperature temperature .Ns]P:p] J09:0390f - IF for for [P:t. J09:0390g - MC1n 1 - [Nu. J09:0390h S NNU hr - .Nu]P:t]Ti:o]S] J09:0390i - YF +. - . J09:0390j - AT the the [S[Np:s. J09:0390k - NN2 tubes tube .Np:s] J09:0390m - YG - - [t118.t118] J09:0390n - VBDR were be [Vwp. J09:0390p - RTn then then [Rsw:G118.Rsw:G118] J09:0400a - VVNt centrifuged centrifuge .Vwp] J09:0400b - II at at [P:h. J09:0400c - MCn 1000 - [Nu. J09:0400d S NNU rpm - .Nu]P:h] J09:0400e - IF for for [P:t. J09:0400f - MC1n 1 - [Nu. J09:0400g S NNU min - .Nu]P:t] J09:0400h - CC and and [S+. J09:0400i - VVNt examined examine [Vn.Vn] J09:0400j - RR macroscopically macroscopically [R:h.R:h] J09:0400k - IF for for [P:r. J09:0410a - NN1n agglutination agglutination .P:r]S+]S] J09:0410b - YF +. - . J09:0410c - IF For for [S[P:c. J09:0410d - AT the the [Ns. J09:0410e - NN1u albumin albumin . J09:0410f - NN1n method method .Ns]P:c] J09:0410g - YC +, - . J09:0410h - JJ equal equal [Np:S119. J09:0410i - NN2 volumes volume . J09:0410j - IO of of [Po. J09:0410k - MCn 30 - [N:121[Nu. J09:0410m - NNUp +% - .Nu] J09:0410n - JJ bovine bovine . J09:0420a - NN1u albumin albumin . J09:0420b - YC +, - . J09:0420c - NN1c sample sample [Ns-.Ns-] J09:0420d - CC and and [Np+. J09:0420e - MCn 2 - [Nu. J09:0420f - NNUp +% - .Nu] J09:0420g - NN2 cells cell . J09:0420h - YG - - [Tn[S121.S121] J09:0420i - VVNt suspended suspend [Vn.Vn] J09:0420j - II in in [P:p. J09:0420k - NN1u saline saline .P:p]Tn]Np+]N:121]Po]Np:S119] J09:0420m - VBDR were be [Vwp. J09:0420n - VVNv allowed allow .Vwp] J09:0420p - YG - - [Ti:o[s119.s119] J09:0420q - TO to to [Vi. J09:0430a - VV0v stand stand .Vi] J09:0430b - II at at [P:p. J09:0430c - NN1n room room [Ns. J09:0430d - NN1n temperature temperature .Ns]P:p] J09:0430e - IF for for [P:t. J09:0430f - MC1n 1 - [Nu. J09:0430g S NNU hr - .Nu]P:t]Ti:o] J09:0430h - CC and and [S+. J09:0430i - RTn then then [Rsw:t.Rsw:t] J09:0430j - VBDR were be [Vwp. J09:0430k - VVNt centrifuged centrifuge .Vwp] J09:0430m - II at at [P:h. J09:0430n - MCn 1000 - [Nu. J09:0440a S NNU rpm - .Nu]P:h] J09:0440b - IF for for [P:t. J09:0440c - MC1n 1 - [Nu. J09:0440d S NNU min - .Nu]P:t]S+]S] J09:0440e - YF +. - . J09:0440f - DBa All all [S[Np:S. J09:0440g - NN2 samples sample .Np:S] J09:0440h - VBDR were be [Vwp. J09:0440i - VVNt tested test .Vwp] J09:0440j - IIb by by [Pb:a. J09:0440k - DB2 both both [Np. J09:0440m - AT the the . J09:0440n - NN1u saline saline [NN1u&. J09:0440p - CC and and [NN1u+. J09:0450a - NN1u albumin albumin .NN1u+]NN1u&] J09:0450b - NN2 methods method .Np]Pb:a]S] J09:0450c - YF +. - . J09:0450d - AT The the [S[Np:S. J09:0450e - NN2 activities activity . J09:0450f - IO of of [Po. J09:0450g - NN2 fractions fraction [Np. J09:0450h - IO of of [Po. J09:0450i - NN2 sera serum [Np:123. J09:0450j - YG - - [Tg[s123.s123] J09:0450k - VVGt containing contain [Vg.Vg] J09:0450m S FWs Rh - [Np:o[Nn.Nn] J09:0460a - NN2 antibodies antibody .Np:o]Tg]Np:123]Po]Np]Po]Np:S] J09:0460b - VBDR were be [Vwp. J09:0460c - VVNt tested test .Vwp] J09:0460d - IIb by by [Pb:a. J09:0460e - AT the the [Np. J09:0460f - NN1u saline saline [N. J09:0460g - YC +, - . J09:0460h - NN1u albumin albumin [Ns-.Ns-] J09:0460i - CC and and [Nns+. J09:0460j - JJ indirect indirect . J09:0460k - NP1s Coombs Coombs .Nns+]N] J09:0470a - NN2 techniques technique .Np]Pb:a]S] J09:0470b - YF +. - . J09:0470c - JJ Homozygous homozygous [S[Np:s[JJ&. J09:0470d - CC and and [JJ+. J09:0470e - JJ heterozygous heterozygous .JJ+]JJ&] J09:0470f - FO - . J09:0470g - NN2 cells cell . J09:0470h - YC +, - . J09:0470i - FO - [Np-. J09:0470j - CC and and . J09:0470k - JJ homozygous homozygous [JJ&. J09:0480a - CC and and [JJ+. J09:0480b - JJ heterozygous heterozygous .JJ+]JJ&] J09:0480c - FO - . J09:0480d - NN2 cells cell .Np-]Np:s] J09:0480e - VBDR were be [Vwp. J09:0480f - VVNt used use .Vwp] J09:0480g - TO to to [Ti:c[Vi. J09:0480h - VV0t test test .Vi] J09:0480i - DD1q each each [Ns:o. J09:0480j - NN1c sample sample .Ns:o]Ti:c] J09:0480k - YS +; - . J09:0480m - RR however however [S-[R:c.R:c] J09:0480n - YC +, - . J09:0481a - II in in [P:c. J09:0481b - AT the the [Ns. J09:0490a - NN1n interest interest . J09:0490b - IO of of [Po. J09:0490c - NN1u clarity clarity [NN1u&. J09:0490d - CC and and [NN1u+. J09:0490e - NN1u conciseness conciseness .NN1u+]NN1u&]Po]Ns]P:c] J09:0490f - RRx only only [R:m.R:m] J09:0490g - AT the the [Np:S125. J09:0490h - NN2 results result . J09:0490i - YG - - [Tn[S125.S125] J09:0490j - VVNv obtained obtain [Vn.Vn] J09:0490k - IW with with [P:h. J09:0500a - JJ homozygous homozygous [Np[N. J09:0500b - FO - . J09:0500c - CC and and [N+. J09:0500d - JJ homozygous homozygous . J09:0500e - FO - .N+]N] J09:0500f - NN2 cells cell .Np]P:h]Tn]Np:S125] J09:0500g - VMo will will [Vcp. J09:0500h - VB0 be be . J09:0500i - VVNt presented present .Vcp] J09:0500j - RLh here here [Rw:p.Rw:p]S-]S] J09:0500k - YF +. - . J09:0510a - AT The the [S[Np:S. J09:0510b - NN1u saline saline [NN1u&. J09:0510c - CC and and [NN1u+. J09:0510d - NN1u albumin albumin .NN1u+]NN1u&] J09:0510e - NN2 tests test .Np:S] J09:0510f - VBDR were be [Vwp. J09:0510g - VVNv performed perform .Vwp] J09:0510h - CSA as as [A:h. J09:0510i - VVNt described describe [Vn.Vn] J09:0510j - IF for for [P:r. J09:0510k - AT the the [Np. J09:0510m S FOs ABO - . J09:0520a - NN2 samples sample .Np]P:r]A:h] J09:0520b - CS21 except except [Fa:m[CS=. J09:0520c - CS22 that that .CS=] J09:0520d - AT the the [Ns:S. J09:0520e - NN1n mixture mixture .Ns:S] J09:0520f - VBDZ was be [Vsp. J09:0520g - VVNv incubated incubate .Vsp] J09:0520h - IF for for [P:t. J09:0520i - MC1n 1 - [Nu. J09:0520j S NNU hr - .Nu]P:t] J09:0520k - II at at [P:p. J09:0520m - MCn 37 - [Nu. J09:0520n S NNU +C - .Nu]P:p] J09:0530a - ICSt before before [P:t. J09:0530b - NN1u centrifugation centrifugation .P:t]Fa:m]S] J09:0530c - YF +. - . J09:0530d - AT The the [S[Np:S. J09:0530e - NN1u saline saline . J09:0530f - NN2 tubes tube .Np:S] J09:0530g - VBDR were be [Vwp. J09:0530h - VVNv saved save [VVNv&. J09:0530i - CC and and [VVNt+. J09:0530j - VVNt used use .VVNt+]VVNv&]Vwp] J09:0531a - IF for for [P:c. J09:0540a - AT the the [Ns. J09:0540b - JJ indirect indirect . J09:0540c - NP1s Coombs Coombs [Nns.Nns] J09:0540d - NN1c test test .Ns]P:c] J09:0540e - II in in [P:h. J09:0540f - AT the the [Ns. J09:0540g - JJ following following . J09:0540h - NN1c manner manner .Ns]P:h]S] J09:0540i - YF +. - . J09:0540j - AT The the [S[Np:S. J09:0540k - NN2 cells cell .Np:S] J09:0540m - VBDR were be [Vwp. J09:0540n - VVNv washed wash .Vwp] J09:0550a - MC three three [Np:t. J09:0550b - NNT2 times time .Np:t] J09:0550c - IW with with [P:h. J09:0550d - NN1u saline saline .P:h] J09:0550e - YC +, - . J09:0550f - FB anti anti [S-[Ns:S[J. J09:0550g - YH + - . J09:0550h - JJ +human human .J] J09:0550i - NN1u serum serum .Ns:S] J09:0550j - VBDZ was be [Vsp. J09:0550k - VVNv added add .Vsp]S-] J09:0550m - YC +, - . J09:0550n - AT the the [S-[Np:S. J09:0550p - NN2 cells cell .Np:S] J09:0550q - VBDR were be [Vwp. J09:0560a - VVNt resuspended resuspend .Vwp]S-] J09:0560b - YC +, - . J09:0560c - CC and and [S+. J09:0560d - AT the the [Ns:S. J09:0560e - NN1n mixture mixture .Ns:S] J09:0560f - VBDZ was be [Vsp. J09:0560g - VVNt centrifuged centrifuge .Vsp] J09:0560h - II at at [P:h. J09:0560i - MCn 1000 - [Nu. J09:0560j S NNU rpm - .Nu]P:h] J09:0560k - IF for for [P:t. J09:0560m - MC1n 1 - [Nu. J09:0560n S NNU min - .Nu]P:t] J09:0570a - CC and and [S+. J09:0570b - VVNt examined examine [Vn.Vn] J09:0570c - IF for for [P:r. J09:0570d - NN1u agglutination agglutination .P:r]S+]S+]S] J09:0570e - YF +. - . J09:0570f - AT The the [S[Np:S127. J09:0570g - FB anti anti [J. J09:0570h - YH + - . J09:0570i - JJ +human human .J] J09:0570j - NN2 sera serum . J09:0570k - YG - - [Tn[S127.S127] J09:0570m - VVNt used use [Vn.Vn]Tn]Np:S127] J09:0570n - VBDR were be [Vwp. J09:0570p - VVNv prepared prepare .Vwp] J09:0580a - IIb by by [Pb:q. J09:0580b - VVGt injecting inject [Tg[Vg.Vg] J09:0580c - JJ whole whole [Ns:o. J09:0580d - JJ human human . J09:0580e - NN1u serum serum .Ns:o] J09:0580f - II into into [P:q. J09:0580g - NN2 rabbits rabbit .P:q]Tg]Pb:q]S] J09:0580h - YF +. - . J09:0580i - DD2a Those those [S[Np:S129. J09:0580j - NN2 antisera antiserum . J09:0580k - YG - - [Tn[S129.S129] J09:0580m - VVNv shown show [Vn.Vn] J09:0590a - IIb by by [Pb:h. J09:0590b - NN1u immunoelectrophoresis immunoelectrophoresis .Pb:h] J09:0590c - YG - - [Ti:o[s129.s129] J09:0590d - TO to to [Vib. J09:0590e - VB0 be be .Vib] J09:0590f - IO of of [Po:e. J09:0590g - AT the the [Ns. J09:0590h - YIL - . J09:0590i - JJ +broad broad [Ns. J09:0590j - NN1c spectrum spectrum .Ns] J09:0590k - YIR + - . J09:0590m - NN1n type type .Ns]Po:e]Ti:o]Tn]Np:S129] J09:0590n - VBDR were be [Vwp. J09:0600a - VVNt selected select .Vwp] J09:0600b - IF for for [P:c. J09:0600c E NN1n used use [Ns. J09:0600d - II in in [P. J09:0600e - AT the the [Ns. J09:0600f - JJ present present . J09:0600g - NN1n study study .Ns]P]Ns]P:c]S] J09:0600h - YF +. - .O] J09:0600i - YB - [Oh.Oh] J09:0600j - AT The the [O[S[Np:S. J09:0600k - JJ red red . J09:0600m - NN2 cells cell . J09:0600n - IF for for [P. J09:0600p - AT the the [Np. J09:0610a S FWs Rh - [Ns[Nn.Nn] J09:0610b - NN1c antibody antibody .Ns] J09:0610c - NN2 tests test .Np]P]Np:S] J09:0610d - VBDR were be [Vwp. J09:0610e - VVNt used use .Vwp] J09:0610f - II within within [P:t. J09:0610g - MCn 3 - [Np. J09:0610h - NNT2 days day . J09:0610i - ICSt after after [P. J09:0610j - VVGv drawing draw [Tg[Vg.Vg]Tg]P]Np]P:t] J09:0610k - II21 except except [P:m[II=. J09:0620a - II22 for for .II=] J09:0620b - AT the the [Np:131. J09:0620c - FO - . J09:0620d - NN2 cells cell . J09:0620e - YC +, - . J09:0620f - DDQr which which [Fr[Dq:S131.Dq:S131] J09:0620g - VHD had have [Vdfp. J09:0620h - VBN been be . J09:0620i - VVNt glycerolized glycerolize .Vdfp] J09:0620j - CC and and [Fr+. J09:0620k - VVNt stored store [Vn.Vn] J09:0620m - II at at [P:p. J09:0620n - MCn 20 - [Nu. J09:0620p S NNU +C - .Nu]P:p] J09:0630a - IF for for [P:t. J09:0630b - RR approximately approximately [Ns[Ms. J09:0630c - MC1n 1 - .Ms] J09:0630d - NNT1c year year .Ns]P:t]Fr+]Fr]Np:131]P:m]S] J09:0630e - YF +. - . J09:0630f - DD2i These these [S[Np:S. J09:0630g - NN2 cells cell .Np:S] J09:0630h - VBDR were be [Vwp. J09:0630i - VVNv thawed thaw .Vwp] J09:0630j - II at at [P:p. J09:0630k - MCn 37 - [Nu. J09:0630m S NNU +C - .Nu]P:p] J09:0630n - IF for for [P:t. J09:0630p - MCn 30 - [Nu. J09:0640a S NNU min - .Nu]P:t] J09:0640b - CC and and [S+. J09:0640c - VBDR were be [Vwp. J09:0640d - VVNt deglycerolized deglycerolize .Vwp] J09:0640e - IIb by by [Pb:h. J09:0640f - RR alternately alternately . J09:0640g - VVGt centrifuging centrifuge [Tg[Vg.Vg] J09:0640h - CC and and [Tg+. J09:0640i - VVGv mixing mix [Vg.Vg] J09:0650a - IW with with [P:w. J09:0650b - VVGv descending descend [Np[Tg[Vg.Vg]Tg] J09:0650c - NN2 concentrations concentration . J09:0650d - IO of of [Po. J09:0650e - NN1u glycerol glycerol [Np. J09:0650f - NN2 solutions solution . J09:0650g - YPL ( - . J09:0650h - MCn +20 - [Nu@[MCn&. J09:0650i - YC +, - . J09:0650j - MCn 18 - [MCn-.MCn-] J09:0650k - YC +, - . J09:0650m - MCn 10 - [MCn-.MCn-] J09:0650n - YC +, - . J09:0650p - MCn 8 - [MCn-.MCn-] J09:0650q - YC +, - . J09:0650r - MCn 4 - [MCn-.MCn-] J09:0660a - CC and and [MCn+. J09:0660b - MCn 2 - .MCn+]MCn&] J09:0660c - NNUp +% - .Nu@] J09:0660d - YPR +) - .Np]Po]Np]P:w]Tg+]Tg]Pb:h]S+]S] J09:0660e - YF +. - . J09:0660f - AT The the [S[Np:S. J09:0660g - NN2 cells cell .Np:S] J09:0660h - YG - - [t133.t133] J09:0660i - VBDR were be [Vwp. J09:0660j - RTn then then [Rsw:G133.Rsw:G133] J09:0660k - VVNv washed wash .Vwp] J09:0660m - MC three three [Np:t. J09:0660n - NNT2 times time .Np:t] J09:0660p - IW with with [P:h. J09:0660q - NN1u saline saline .P:h] J09:0660r - CC and and [S+. J09:0660s - VVNt resuspended resuspend [Vn.Vn] J09:0670a - IIt to to [P:q. J09:0670b - MCn 2 - [Nu. J09:0670c - NNUp +% - .Nu]P:q] J09:0670d - II in in [P:p. J09:0670e - NN1u saline saline .P:p]S+]S] J09:0670f - YF +. - .O] J09:0670g - YBL - [Oh. J09:0670h - NN1u CHROMATOGRAPHY chromatography . J09:0670i - YF +. - . J09:0670j - YBR - .Oh] J09:0670k - NN1n Blood blood [O[S[Np:S135. J09:0670m - NN2 samples sample .Np:S135] J09:0670n - VBDR were be [Vwp. J09:0680a - VVNv allowed allow .Vwp] J09:0680b - YG - - [Ti:o[s135.s135] J09:0680c - TO to to [Vi. J09:0680d - VV0v clot clot .Vi] J09:0680e - II at at [P:p. J09:0680f - NN1n room room [Ns. J09:0680g - NN1n temperature temperature .Ns]P:p] J09:0680h - IF for for [P:t. J09:0680i - MCn 3 - [Nu. J09:0680j S NNU hr - .Nu]P:t]Ti:o] J09:0680k - YC +, - . J09:0680m - VVNt centrifuged centrifuge [S-[Vn.Vn]S-] J09:0680n - CC and and [S+. J09:0680p - AT the the [Ns:S. J09:0681a - NN1u serum serum .Ns:S] J09:0690a - VBDZ was be [Vsp. J09:0690b - VVNv removed remove .Vsp]S+]S] J09:0690c - YF +. - . J09:0690d - AT The the [S[Ns:S. J09:0690e - NN1u serum serum .Ns:S] J09:0690f - VBDZ was be [Vsp. J09:0690g - VVNv measured measure .Vsp] J09:0690h - RR volumetrically volumetrically [R:h.R:h] J09:0690i - CC and and [S+. J09:0690j - RR subsequently subsequently [R:t.R:t] J09:0700a - VVNt dialyzed dialyse [Vn.Vn] J09:0700b - II in in [P:p. J09:0700c - AT the the [Ns. J09:0700d - NN1n cold cold .Ns]P:p] J09:0700e - IF for for [P:t. J09:0700f - RR21 at at [RR=. J09:0700g - RR22 least least .RR=] J09:0700h - MCn 24 - [Nu. J09:0700i S NNU hr - .Nu]P:t] J09:0700j - II against against [P:h. J09:0700k - MC three three [Np:136[M. J09:0700m - IIt to to [P. J09:0700n - MC four four .P]M] J09:0700p - NN2 changes change . J09:0700q - YC +, - . J09:0700r - YG - - [L[s136.s136] J09:0710a - RR approximately approximately [Nu:e. J09:0710b - MCn 750 - . J09:0710c S NNU ml - .Nu:e] J09:0710d - RAq each each [R:h.R:h]L] J09:0710e - YC +, - . J09:0710f - IO of of [Po. J09:0710g - YIL - . J09:0710h - VVGv +starting start [Ns[Tg[Vg.Vg]Tg] J09:0710i - NN1c buffer buffer .Ns] J09:0710j - YIR + - .Po]Np:136]P:h]S+]S] J09:0710k - YF +. - . J09:0710m - DD1i This this [S[Ns:s. J09:0720a - NN1c buffer buffer . J09:0720b - YC +, - . J09:0720c S NNUb pH - [Nu@. J09:0720d - MCd 8.6 - .Nu@] J09:0720e - YC +, - .Ns:s] J09:0720f - VBDZ was be [Vsb.Vsb] J09:0720g - MCd 0.005 - [Nu:e. J09:0720h S NNU M - . J09:0720i - II in in [P. J09:0720j - FO - .P] J09:0720k - CC and and [Nu+. J09:0720m - MCd 0.039 - . J09:0720n S NNU M - . J09:0720p - II in in [P. J09:0725a - NN1u tris(hydroxymethyl)aminomethane tris(hydroxymethyl)aminomethane [Ns. J09:0730a - YPL ( - . J09:0730b - NN1u +Tris Tris . J09:0730c - YPR +) - .Ns]P]Nu+]Nu:e]S] J09:0730d - YF +. - . J09:0730e - ICSt After after [S[P:t. J09:0730f - NN1u dialysis dialysis .P:t] J09:0730g - AT the the [Ns:S. J09:0730h - NN1c sample sample .Ns:S] J09:0730i - VBDZ was be [Vsp. J09:0730j - VVNt centrifuged centrifuge .Vsp] J09:0740a - CC and and [S+. J09:0740b - AT the the [Ns:s. J09:0740c - NN1n supernatant supernatant .Ns:s] J09:0740d - VVNt placed place [Vn.Vn] J09:0740e - II on on [P:q. J09:0740f - AT1 a a [Ns. J09:0740g - FO - [Nu. J09:0740h S NNU cm - .Nu] J09:0740i - NN1c column column . J09:0740j - IO of of [Po. J09:0740k S FOc DEAE - [Ns:137. J09:0740m - YH + - . J09:0740n - NN1u +cellulose cellulose . J09:0740p - YG - - [Tn[S137.S137] J09:0750a - VVNt equilibrated equilibrate [Vn.Vn] J09:0750b - IW with with [P:h. J09:0750c - VVGv starting start [Ns[Tg[Vg.Vg]Tg] J09:0750d - NN1c buffer buffer .Ns]P:h]Tn]Ns:137]Po]Ns]P:q]S+]S] J09:0750e - YF +. - . J09:0750f - AT The the [S[Ns:S139. J09:0750g S FOc DEAE - [Ns. J09:0750h - YH + - . J09:0750i - NN1u +cellulose cellulose .Ns] J09:0750j - YC +, - . J09:0750k - YG - - [Tg[s139.s139] J09:0760a - VVGt containing contain [Vg.Vg] J09:0760b - MCd 0.78 - [Nu:o. J09:0760c S NNU mEq - . J09:0760d - IO of of [Po. J09:0760e S FOc N - [Nu. J09:0760f - IIp + - [P. J09:0760g S NNU +g - .P]Nu]Po]Nu:o]Tg] J09:0760h - YC +, - .Ns:S139] J09:0760i - VBDZ was be [Vsp. J09:0760j - VVNv prepared prepare .Vsp] J09:0760k - II in in [P:p. J09:0760m - APPGi2 our our [Ns. J09:0760n - NN1c laboratory laboratory .Ns]P:p] J09:0770a - IIb by by [Pb:h. J09:0770b - AT the the [Ns. J09:0770c - NN1n method method . J09:0770d - IO of of [Po. J09:0770e - NP1s Peterson Peterson [Nn[NP1s&. J09:0770f - CC and and [NP1s+. J09:0770g - NP1s Sober Sober .NP1s+]NP1s&] J09:0770h - YPL ( - . J09:0770i - MCn +7 - [Iu.Iu] J09:0770j - YPR +) - .Nn]Po]Ns]Pb:h] J09:0770k - II from from [P:h. J09:0770m - JJ powdered powdered [Ns. J09:0770n - NN1u cellulose cellulose . J09:0770p - YC +, - . J09:0770q - MCn 100 - [Ns@. J09:0770r - YH + - [P. J09:0770s - MCn +230 - .P] J09:0780a - NN1c mesh mesh .Ns@]Ns]P:h]S] J09:0780b - YF +. - . J09:0780c - AT The the [S[Ns:S141. J09:0780d - JJ small small . J09:0780e - NN1c amount amount . J09:0780f - IO of of [Po. J09:0780g - JJ insoluble insoluble [Ns. J09:0780h - NN1n material material .Ns]Po] J09:0780i - DDQr which which [Fr[Dq:s141.Dq:s141] J09:0780j - VVDt precipitated precipitate [Vd.Vd] J09:0790a - II during during [P:t. J09:0790b - NN1u dialysis dialysis .P:t]Fr]Ns:S141] J09:0790c - VBDZ was be [Vsp. J09:0790d - VVNt suspended suspend .Vsp] J09:0790e - II in in [P:p. J09:0790f - RR approximately approximately [Nu[M. J09:0790g - MCn 5 - .M] J09:0790h S NNU ml - . J09:0790i - IO of of [Po. J09:0790j - VVGv starting start [Ns[Tg[Vg.Vg]Tg] J09:0790k - NN1c buffer buffer .Ns]Po]Nu]P:p] J09:0790m - YC +, - . J09:0800a - VVNt centrifuged centrifuge [S-[Vn.Vn]S-] J09:0800b - YC +, - . J09:0800c - VVNt resuspended resuspend [S-[Vn.Vn] J09:0800d - II in in [P:p. J09:0800e - MCd 2.5 - [Nu. J09:0800f S NNU ml - . J09:0800g - IO of of [Po. J09:0800h - JJ isotonic isotonic [Ns. J09:0800i - NN1u saline saline .Ns]Po]Nu]P:p]S-] J09:0800j - CC and and [S+. J09:0800k - VVNt tested test [Vn.Vn] J09:0810a - IF for for [P:r. J09:0810b - NN1c antibody antibody [Ns. J09:0810c - NN1n activity activity .Ns]P:r]S+]S] J09:0810d - YF +. - .O] J09:0810e - YB - [Oh.Oh] J09:0810f - AT The the [O[S[Ns:s. J09:0810g - NN1u chromatography chromatography .Ns:s] J09:0810h - VBDZ was be [Vsp. J09:0810i - VDN done do .Vsp] J09:0810j - II at at [P:p. J09:0810k - MCn 6 - [Nu. J09:0810m S NNU +C - .Nu]P:p] J09:0820a - VVGt using use [Tg:h[Vg.Vg] J09:0820b - NN1c gradient gradient [Ns:o. J09:0820c - NN1n elution elution .Ns:o] J09:0820d - YC +, - . J09:0820e - RR essentially essentially [R:m.R:m] J09:0820f - II21 according accord [P:h[II=. J09:0820g - II22 to to .II=] J09:0820h - NP1s Sober Sober [Nn[NP1s&. J09:0820i - CC and and [NP1s+. J09:0820j - NP1s Peterson Peterson .NP1s+]NP1s&]Nn]P:h]Tg:h]S] J09:0820k - YF +. - . J09:0830a - AT The the [S[Ns:S143. J09:0830b - JJ deep deep . J09:0830c - JJ concave concave . J09:0830d - NN1c gradient gradient . J09:0830e - YG - - [Tn[S143.S143] J09:0830f - VVNt employed employ [Vn.Vn]Tn] J09:0830g - YPL ( - . J09:0830h A NNm +fig. - [Iu[Nns. J09:0830i - MCn 2 - .Nns]Iu] J09:0830j - YPR +) - .Ns:S143] J09:0830k - VBDZ was be [Vsp. J09:0830m - VVNv obtained obtain .Vsp] J09:0830n - IW with with [P:h. J09:0840a - AT1 a a [Ns. J09:0840b - MC nine nine [J. J09:0840c - YH + - . J09:0840d - JJh +chambered chamber .J] J09:0840e - NN1c gradient gradient . J09:0840f - NN1n elution elution . J09:0840g - NN1c device device . J09:0840h - YPL ( - . J09:0840i - YIL + - [Nns@. J09:0840j - NP1x +Varigrad Varigrad . J09:0840k - YIR + - . J09:0840m - YC +, - . J09:0840n - NN1n reference reference [Iu[Nns. J09:0850a - MCb (8) - .Nns]Iu]Nns@] J09:0850b - YPR +) - .Ns]P:h] J09:0850c - CC and and [S+. J09:0850d - VHZ has have [Vzfp. J09:0850e - VBN been be . J09:0850f - VVNt described describe .Vzfp] J09:0850g - RLe elsewhere elsewhere [Rw:p.Rw:p]S+]S] J09:0850h - YF +. - . J09:0850i - AT The the [S[Nj:S. J09:0850j - JBo other other . J09:0850k - YC +, - . J09:0850m - AT1 a a [Ns@. J09:0850n - JJ shallow shallow . J09:0850p - JJ concave concave . J09:0860a - NN1c gradient gradient . J09:0860b - YPL ( - . J09:0860c A NNm +Fig. - [Iu[Nns. J09:0860d - MC1n 1 - .Nns]Iu] J09:0860e - YPR +) - .Ns@] J09:0860f - YC +, - .Nj:S] J09:0860g - VBDZ was be [Vsp. J09:0860h - VVNv produced produce .Vsp] J09:0860i - IW with with [P:h. J09:0860j - AT1 a a [Ns. J09:0860k - JB socalled socalled . J09:0860m - YIL - . J09:0860n - NN1c +cone cone [N. J09:0860p - YH + - . J09:0860q - NN1c +sphere sphere .N] J09:0860r - YIR + - . J09:0870a - NN1n apparatus apparatus .Ns]P:h] J09:0870b - YC +, - . J09:0870c - AT the the [Tg:b[Ns:s. J09:0870d - YIL - . J09:0870e - NN1c +cone cone . J09:0870f - YIR + - .Ns:s] J09:0870g - VBG being be [Vgb.Vgb] J09:0870h - AT1 a a [Ns:e. J09:0870i - MCn 2 - [Nu. J09:0870j - YH + - . J09:0870k - NNU1c +liter litre .Nu] J09:0870m - NP1s Erlenmeyer Erlenmeyer [Nns.Nns] J09:0870n - NN1c flask flask .Ns:e] J09:0880a - CC and and [Tg+. J09:0880b - AT the the [Ns:s. J09:0880c - YIL - . J09:0880d - NN1c +sphere sphere .Ns:s] J09:0880e - YC +, - . J09:0880f - YIR + - . J09:0880g - AT1 a a [Ns:e. J09:0880h - MCn 2 - [Nu. J09:0880i - YH + - . J09:0880j - NNU1c +liter litre .Nu] J09:0880k - JJ round round [Ns. J09:0880m - YH + - . J09:0880n - NN1c +bottom bottom .Ns] J09:0880p - NN1c flask flask .Ns:e]Tg+]Tg:b]S] J09:0880q - YF +. - . J09:0880r - DD1q Each each [S[Ds:s.Ds:s] J09:0880s - RR initially initially [R:t.R:t] J09:0880t - VVDt contained contain [Vd.Vd] J09:0890a - MCy 1700 - [Nu:o. J09:0890b S NNU ml - . J09:0890c - IO of of [Po. J09:0890d - NN1c buffer buffer .Po]Nu:o] J09:0890e - YS +; - . J09:0890f - II in in [S@[P:p. J09:0890g - AT the the [Ns. J09:0890h - NN1c sphere sphere .Ns]P:p] J09:0890i - VBDZ was be [Vsb.Vsb] J09:0890j - VVGv starting start [Ns:s[Tg[Vg.Vg]Tg] J09:0890k - NN1c buffer buffer .Ns:s] J09:0890m - CC and and [S+. J09:0900a - II in in [P:p. J09:0900b - AT the the [Ns. J09:0900c - NN1c cone cone .Ns]P:p] J09:0900d - VBDZ was be [Vsb.Vsb] J09:0900e - JJ final final [Ns:s. J09:0900f - NN1c buffer buffer .Ns:s] J09:0900g - YC +, - . J09:0900h - MCd 0.50 - [L:b[Nu:s. J09:0900i S NNU M - .Nu:s] J09:0900j - II in in [P:r. J09:0900k - DB2 both both [N. J09:0900m - FOc - [FOc&. J09:0900n - CC and and [NN1u+. J09:0900p - NN1u Tris Tris .NN1u+]FOc&]N]P:r] J09:0900q - YC +, - . J09:0900r S NNUb pH - [Nu@. J09:0901a - MCd 4.1 - .Nu@]L:b]S+]S@]S] J09:0901b - YF +. - .O] J09:0910a - YB - [Oh.Oh] J09:0910b - AT1 A a [O[S[Ns:S. J09:0910c - NN1c flow flow . J09:0910d - NN1c rate rate . J09:0910e - IO of of [Po. J09:0910f - MCn 72 - [N. J09:0910g - FO - .N]Po]Ns:S] J09:0910h - VBDZ was be [Vsp. J09:0910i - VVNt used use .Vsp] J09:0910j - CC and and [S+. J09:0910k - MCn 12 - [Np:S[Nu. J09:0910m S NNU ml - .Nu] J09:0910n - NN2 fractions fraction .Np:S] J09:0910p - VBDR were be [Vwp. J09:0910q - VVNv collected collect .Vwp]S+]S] J09:0910r - YF +. - . J09:0920a - RR Approximately approximately [S[Np:S[M. J09:0920b - MCn 165 - .M] J09:0920c - NN2 fractions fraction .Np:S] J09:0920d - VBDR were be [Vwp. J09:0920e - VVNv obtained obtain .Vwp] J09:0920f - II from from [P:q. J09:0920g - DD1q each each [Ns. J09:0920h - NN1c column column .Ns]P:q]S] J09:0920i - YF +. - . J09:0930a - DD2i These these [S[Dp:S.Dp:S] J09:0930b - VBDR were be [Vwp. J09:0930c - VVNv read read .Vwp] J09:0930d - II at at [P:p. J09:0930e - MCn 280 - [Nu. J09:0930f S NNU m - .Nu]P:p] J09:0930g - II in in [P:p. J09:0930h - AT1 a a [Ns. J09:0930i - NP1s Beckman Beckman [Nns.Nns] J09:0930j - NN1c model model [Nns. J09:0930k S NP1z DU - .Nns] J09:0930m - NN1c spectrophotometer spectrophotometer .Ns]P:p] J09:0940a - CC and and [S+. J09:0940b - VVNt tested test [Vn.Vn] J09:0940c - IF for for [P:r. J09:0940d - NN1c antibody antibody [Ns. J09:0940e - NN1n activity activity .Ns]P:r] J09:0940f - CSA as as [A:h. J09:0940g - VVNt described describe [Vn.Vn] J09:0940h - RL above above [R:p.R:p]A:h]S+]S] J09:0940i - YF +. - .O] J09:0940j - YBL - [Oh. J09:0940k - NN1n PAPER paper [Ns. J09:0950a - NN1u ELECTROPHORESIS electrophoresis .Ns] J09:0950b - YF +. - . J09:0950c - YBR - .Oh] J09:0950d - IF For for [O[S[P:c. J09:0950e - NN1n protein protein [Ns. J09:0950f - NN1u identification identification .Ns]P:c] J09:0950g - YC +, - . J09:0950h - NN2 fractions fraction [Np:S. J09:0950i - II from from [P. J09:0950j - AT the the [Ns. J09:0960a - NN1c column column .Ns]P]Np:S] J09:0960b - VBDR were be [Vwp. J09:0960c - VVNv concentrated concentrate .Vwp] J09:0960d - IIb by by [Pb:h. J09:0960e - NN1u pervaporation pervaporation [Ns. J09:0960f - II against against [P. J09:0960g - AT1 a a [Ns. J09:0960h - NN1c stream stream . J09:0960i - IO of of [Po. J09:0960j - NN1n air air .Po]Ns]P] J09:0960k - II at at [P. J09:0960m - MCn 5 - [Nu. J09:0960n S NNU +C - .Nu]P]Ns] J09:0970a - CCr or or [Pb+. J09:0970b - IIb by by . J09:0970c - JJ negative negative [Ns[Ns. J09:0970d - NN1n pressure pressure .Ns] J09:0970e - NN1u dialysis dialysis . J09:0970f - II in in [P. J09:0970g - AT1 an an [Ns:145. J09:0970h - NN1n apparatus apparatus . J09:0970i - DDQr which which [Fr[Dq:s145.Dq:s145] J09:0970j - VVDv permitted permit [Vd.Vd] J09:0980a - JJ simultaneous simultaneous [N:o. J09:0980b - NN1n concentration concentration . J09:0980c - IO of of [Po. J09:0980d - AT the the [Ns. J09:0980e - NN1n protein protein .Ns]Po] J09:0980f - CC and and [Ns+. J09:0980g - NN1u dialysis dialysis . J09:0980h - II against against [P. J09:0980i - JJ isotonic isotonic [Ns. J09:0990a - NN1u saline saline .Ns]P]Ns+]N:o]Fr]Ns:145]P]Ns]Pb+]Pb:h]S] J09:0990b - YF +. - . J09:0990c - II During during [S[P:t. J09:0990d - AT the the [Ns. J09:0990e - DAr latter latter . J09:0990f - NN1n procedure procedure .Ns]P:t] J09:0990g - AT the the [Ns:S. J09:0990h - NN1n temperature temperature .Ns:S] J09:0990i - VBDZ was be [Vsp. J09:0990j - VVNt maintained maintain .Vsp] J09:1000a - II at at [P:p. J09:1000b - MCn 2 - [Nu. J09:1000c S NNU +C - .Nu]P:p] J09:1000d - IIb by by [Pb:h. J09:1000e - VVGt surrounding surround [Tg[Vg.Vg] J09:1000f - AT the the [Ns:o. J09:1000g - NN1n apparatus apparatus .Ns:o] J09:1000h - IW with with [P:h. J09:1000i - NN1n ice ice .P:h]Tg]Pb:h]S] J09:1000j - YF +. - . J09:1000k - CS Because because [S[Fa:c. J09:1000m - JJ negative negative [Ns:s[Ns. J09:1001a - NN1n pressure pressure .Ns] J09:1010a - NN1u dialysis dialysis .Ns:s] J09:1010b - VVDv gave give [Vd.Vd] J09:1010c - JJR better good [Ns:o. J09:1010d - NN1n recovery recovery . J09:1010e - IO of of [Po. J09:1010f - NN2 proteins protein .Po]Ns:o] J09:1010g - YC +, - . J09:1010h - VVDv permitted permit [Fa-[Vd.Vd] J09:1010i - NN1u detection detection [Ns:o. J09:1020a - IO of of [Po. J09:1020b - NN2 proteins protein [Np:147. J09:1020c - YG - - [Tn[S147.S147] J09:1020d - VVNv concentrated concentrate [Vn.Vn] J09:1020e - II from from [P:h. J09:1020f - RG very very [Np[J. J09:1020g - JJ dilute dilute .J] J09:1020h - NN2 solutions solution .Np]P:h]Tn]Np:147]Po]Ns:o]Fa-] J09:1020i - CC and and [Fa+. J09:1020j - VBDZ was be [Vsb.Vsb] J09:1020k - AT1 a a [Ns:e. J09:1020m - JJR gentler gentle . J09:1030a - NN1n procedure procedure .Ns:e]Fa+]Fa:c] J09:1030b - YC +, - . J09:1030c - PPH1 it it [Ni:S.Ni:S] J09:1030d - VBDZ was be [Vsp. J09:1030e - VVNt used use .Vsp] J09:1030f - II in in [P:p. J09:1030g - DBa all all [Np[D. J09:1030h - ICSx but but [P. J09:1030i - AT the the [Nj. J09:1030j - JJT earliest early .Nj]P]D] J09:1030k - NN2 experiments experiment .Np]P:p]S] J09:1030m - YF +. - .O] J09:1030n - YB - [Oh.Oh] J09:1030p - NN1n Paper paper [O[S[Ns:S. J09:1040a - NN1u electrophoresis electrophoresis .Ns:S] J09:1040b - VBDZ was be [Vsp. J09:1040c - VVNv carried carry .Vsp] J09:1040d - RP out out [R:n.R:n] J09:1040e - II on on [P:u. J09:1040f - AT the the [Np. J09:1040g - JJ concentrated concentrated . J09:1040h - NN2 samples sample .Np]P:u] J09:1040i - II in in [P:p. J09:1040j - AT1 a a [Ns. J09:1040k - NP1j Spinco Spinco [Nns.Nns] J09:1050a - NN1c model model [Nns. J09:1050b S FOs R - .Nns] J09:1050c - NN1c cell cell .Ns]P:p] J09:1050d - VVGt using use [Tg:h[Vg.Vg] J09:1050e - NN1u barbital barbital [Ns:o. J09:1050f - NN1c buffer buffer . J09:1050g - YC +, - . J09:1050h S NNUb pH - [Nu@. J09:1050i - MCd 8.6 - .Nu@] J09:1050j - YC +, - . J09:1050k - JJ ionic ionic [L[Ns:s. J09:1050m - NN1n strength strength .Ns:s] J09:1060a - MCd 0.075 - [M:e.M:e]L] J09:1060b - YC +, - .Ns:o] J09:1060c - II at at [P:p. J09:1060d - NN1n room room [Ns. J09:1060e - NN1n temperature temperature .Ns]P:p] J09:1060f - II on on [P:p. J09:1060g - NP1s Whatman Whatman [Ns[Nns.Nns] J09:1060h S FOs 3MM - [Nns.Nns] J09:1060i - NN1c filter filter . J09:1060j - NN1n paper paper .Ns]P:p]Tg:h]S] J09:1060k - YF +. - . J09:1060m - MC Five five [S:149[Nup:S. J09:1070a - NNU2 milliamperes milliampere . J09:1070b - IIp + - [P. J09:1070c - NN1c +cell cell .P]Nup:S] J09:1070d - VBDR were be [Vwp. J09:1070e - VVNv applied apply .Vwp] J09:1070f - IF for for [P:t. J09:1070g - MCn 18 - [Nu. J09:1070h S NNU hr - .Nu]P:t] J09:1070i - YC +, - . J09:1070j - ICSt after after [Fr:x[Pq:t. J09:1070k - DDQr which which [Dq:149.Dq:149]Pq:t] J09:1070m - AT the the [Np:S. J09:1070n - NN2 strips strip .Np:S] J09:1080a - VBDR were be [Vwp. J09:1080b - VVNv stained stain .Vwp] J09:1080c - IW with with [P:h. J09:1080d - NN1u bromphenol bromphenol [J. J09:1080e - JJ blue blue .J]P:h] J09:1080f - CC and and [Fr+. J09:1080g - NN1u densitometry densitometry [Ns:S.Ns:S] J09:1080h - VBDZ was be [Vsp. J09:1080i - VVNv carried carry .Vsp] J09:1080j - RP out out [R:n.R:n] J09:1080k - VVGt using use [Tg:h[Vg.Vg] J09:1090a - AT1 a a [Nns:o. J09:1090b - NP1j Spinco Spinco . J09:1090c - NN1c Analytrol Analytrol .Nns:o]Tg:h]Fr+]Fr:x]S:149] J09:1090d - YF +. - .O] J09:1090e - YB - [Oh.Oh] J09:1090f - CSn When when [O[S[Fa:t[Rq:t.Rq:t] J09:1090g - NN1n paper paper [Ns:s. J09:1090h - NN1u electrophoresis electrophoresis .Ns:s] J09:1090i - VBDZ was be [Vsb.Vsb] J09:1090j - TO to to [Ti:z[Vip. J09:1090k - VB0 be be . J09:1100a - VVNt used use .Vip] J09:1100b - IF for for [P:c. J09:1100c - NN1n preparation preparation .P:c]Ti:z]Fa:t] J09:1100d - YC +, - . J09:1100e - MC eight eight [Np:S. J09:1100f - NN2 strips strip . J09:1100g - IO of of [Po. J09:1100h - AT1 a a [N. J09:1100i - JJ whole whole [Ns. J09:1100j - NN1u serum serum .Ns] J09:1100k - NN1c sample sample . J09:1100m - CCr or or [Ns+:151. J09:1100n - AT1 a a . J09:1100p - JJ chromatographic chromatographic . J09:1110a - NN1c fraction fraction . J09:1110b - YG - - [Tn[S151.S151] J09:1110c - VVNv concentrated concentrate [Vn.Vn] J09:1110d - IIb by by [Pb:h. J09:1110e - JJ negative negative [Ns[Ns. J09:1110f - NN1n pressure pressure .Ns] J09:1110g - NN1u dialysis dialysis .Ns]Pb:h]Tn]Ns+:151]N]Po]Np:S] J09:1110h - VBDR were be [Vwp. J09:1110i - VVNv run run .Vwp] J09:1110j - IIp + - [P:h. J09:1110k - NNL1c +chamber chamber .P:h] J09:1120a - II under under [P:h. J09:1120b - AT the the [Np:153. J09:1120c - NN2 conditions condition . J09:1120d - YG - - [Tn[S153.S153] J09:1120e - VVNt described describe [Vn.Vn] J09:1120f - RL above above [R:p.R:p]Tn]Np:153]P:h]S] J09:1120g - YF +. - . J09:1120h - II At at [S[P:t. J09:1120i - AT the the [Ns. J09:1120j - NN1c end end . J09:1120k - IO of of [Po. J09:1120m - AT the the [Ns. J09:1120n - NN1c run run .Ns]Po]Ns]P:t] J09:1120p - YC +, - . J09:1130a - AT the the [Np:S. J09:1130b - NN2 strips strip . J09:1130c - II in in [P. J09:1130d - AT the the [Np. J09:1130e - MD third third [MD&. J09:1130f - CC and and [MD+. J09:1130g - MD sixth sixth .MD+]MD&] J09:1130h - NN2 positions position . J09:1130i - II in in [P. J09:1130j - DD1q each each [Ns. J09:1130k - NNL1c chamber chamber .Ns]P]Np]P]Np:S] J09:1130m - VBDR were be [Vwp. J09:1130n - VVNv dried dry .Vwp] J09:1130p - YC +, - . J09:1140a - VVNv stained stain [S-[Vn.Vn] J09:1140b - IF for for [P:t. J09:1140c - MC1n 1 - [Nu. J09:1140d S NNU hr - .Nu]P:t]S-] J09:1140e - YC +, - . J09:1140f - VVNv washed wash [S-[Vn.Vn]S-] J09:1140g - CC and and [S+. J09:1140h - VVNv dried dry [Vn.Vn]S+] J09:1140i - YC +, - . J09:1140j - CS while while [Fa:t. J09:1140k - AT the the [Np:S. J09:1140m - JBo other other . J09:1140n - NN2 strips strip .Np:S] J09:1140p - VBDR were be [Vwp. J09:1150a - VVNt maintained maintain .Vwp] J09:1150b - II in in [P:p. J09:1150c - AT1 a a [Ns. J09:1150d - JJ horizontal horizontal . J09:1150e - NN1n position position .Ns]P:p] J09:1150f - II at at [P:p. J09:1150g - MC1n 1 - [Nu. J09:1150h S NNU +C - .Nu]P:p]Fa:t]S] J09:1150i - YF +. - . J09:1150j - AT The the [S[Np:S. J09:1150k - JJ unstained unstained . J09:1150m - NN2 strips strip .Np:S] J09:1150n - YG - - [t155.t155] J09:1151a - VBDR were be [Vwp. J09:1160a - RTn then then [Rsw:G155.Rsw:G155] J09:1160b - VVNt marked mark .Vwp] J09:1160c - YC +, - . J09:1160d - VVGt using use [Tg:b[Vg.Vg] J09:1160e - AT the the [Np:o. J09:1160f - VVNv stained stain [Tn[Vn.Vn]Tn] J09:1160g - MC2 ones one .Np:o] J09:1160h - IIa as as [P:j. J09:1160i - AT1 a a [Ns. J09:1160j - NN1c guide guide .Ns]P:j]Tg:b] J09:1160k - YC +, - . J09:1160m - CC and and [S+. J09:1160n - VVNv cut cut [Vn.Vn] J09:1160p - RR transversely transversely [R:h.R:h] J09:1170a - RRz so so [R:c. J09:1170b - CSA as as [A. J09:1170c - TO to to [Ti:z[Vi. J09:1170d - VV0v separate separate .Vi] J09:1170e - AT the the [Np:o. J09:1170f - JJ various various . J09:1170g - NN1n protein protein . J09:1170h - NN2 bands band .Np:o]Ti:z]A]R:c]S+]S] J09:1170i - YF +. - . J09:1170j - AT The the [S[Np:S157. J09:1170k - NN1c strip strip . J09:1170m - NN2 sections section . J09:1170n - YG - - [Tg[s157.s157] J09:1170p - VVGt containing contain [Vg.Vg] J09:1180a - AT1 a a [Ns:o. J09:1180b - VVNv given give [Tn[Vn.Vn]Tn] J09:1180c - NN1n protein protein .Ns:o]Tg]Np:S157] J09:1180d - VBDR were be [Vwp. J09:1180e - VVNt pooled pool .Vwp] J09:1180f - YC +, - . J09:1180g - VVNt eluted elute [S-[Vn.Vn] J09:1180h - IW with with [P:h. J09:1180i - MCd 0.5 - [Nu. J09:1180j S NNU ml - . J09:1180k - IO of of [Po. J09:1180m - JJ isotonic isotonic [Ns. J09:1190a - NN1u saline saline .Ns]Po]Nu]P:h]S-] J09:1190b - YC +, - . J09:1190c - CC and and [S+. J09:1190d - AT the the [Np:S. J09:1190e - NN2 eluates eluate .Np:S] J09:1190f - VBDR were be [Vwp. J09:1190g - VVNt tested test .Vwp] J09:1190h - IF for for [P:r. J09:1190i - NN1c antibody antibody [Ns. J09:1190j - NN1n activity activity .Ns]P:r]S+]S] J09:1190k - YF +. - .O] J09:1190m - YBL - [Oh. J09:1190n - NN1u ULTRACENTRIFUGATION ultracentrifugation . J09:1190p - YF +. - . J09:1190q - YBR - .Oh] J09:1200a - NN2 Fractions fraction [O[S[Np:S159. J09:1200b - II from from [P. J09:1200c - AT the the [Ns. J09:1200d - NN1c column column .Ns]P] J09:1200e - DDQr which which [Fr[Dq:s159.Dq:s159] J09:1200f - VBDR were be [Vwb.Vwb] J09:1200g - TO to to [Ti:z[Vip. J09:1200h - VB0 be be . J09:1200i - VVNt subjected subject .Vip] J09:1210a - IIt to to [P:u. J09:1210b - JJ analytical analytical [Ns. J09:1210c - NN1u ultracentrifugation ultracentrifugation .Ns]P:u]Ti:z]Fr]Np:S159] J09:1210d - VBDR were be [Vwp. J09:1210e - VVNv concentrated concentrate .Vwp] J09:1210f - IIb by by [Pb:a. J09:1210g - JJ negative negative [Ns. J09:1210h - NN1n pressure pressure . J09:1220a - NN1u dialysis dialysis .Ns]Pb:a] J09:1220b - CC and and [S+. J09:1220c - VVNt dialyzed dialyse [Vn.Vn] J09:1220d - IF for for [P:t. J09:1220e - MCn 16 - [Nu. J09:1220f S NNU hr - .Nu]P:t] J09:1220g - II in in [P:p. J09:1220h - AT the the [Ns. J09:1220i - NN1n cold cold .Ns]P:p] J09:1220j - II against against [P:h. J09:1220k - RR21 at at [Np[RR=. J09:1220m - RR22 least least .RR=] J09:1220n - MCn 500 - . J09:1230a - NN2 volumes volume . J09:1230b - IO of of [Po. J09:1230c - NN1n phosphate phosphate [Ns[J. J09:1230d - YH + - . J09:1230e - JJh +buffered buffer .J] J09:1230f - NN1u saline saline . J09:1230g - YC +, - . J09:1230h S NNUb pH - [Nu@. J09:1230i - MCd 7.2 - .Nu@] J09:1230j - YC +, - . J09:1230k - JJ ionic ionic [L[Ns:s. J09:1230m - NN1n strength strength .Ns:s] J09:1230n - MCd 0.154 - [M:e.M:e]L]Ns]Po]Np]P:h]S+]S] J09:1230p - YF +. - . J09:1240a - PPHS2 They they [S[Nap:S.Nap:S] J09:1240b - YG - - [t161.t161] J09:1240c - VBDR were be [Vwp. J09:1240d - RTn then then [Rsw:G161.Rsw:G161] J09:1240e - VVNt centrifuged centrifuge .Vwp] J09:1240f - II at at [P:h. J09:1240g - MCn 59,780 - [Nu. J09:1240h S NNU rpm - .Nu]P:h] J09:1240i - IF for for [P:t. J09:1240j - MCn 35 - [Nu[M. J09:1240k - IIt to to [P. J09:1240m - MCn 80 - .P]M] J09:1240n S NNU min - .Nu]P:t] J09:1250a - II at at [P:p. J09:1250b - MCn 20 - [Nu. J09:1250c S NNU +C - .Nu]P:p] J09:1250d - II in in [P:p. J09:1250e - AT1 a a [Ns. J09:1250f - NP1j Spinco Spinco [Nns.Nns] J09:1250g - NN1c model model [Nns. J09:1250h S ZZ1 E - .Nns] J09:1250i - NN1c ultracentrifuge ultracentrifuge .Ns]P:p] J09:1250j - II at at [P:p. J09:1250k - AT1 a a [Ns. J09:1250m - NN1n protein protein . J09:1250n - NN1n concentration concentration . J09:1260a - IO of of [Po. J09:1260b - MCd 1.00 - [Nu[M. J09:1260c - IIt to to [P. J09:1260d - MCd 1.25 - .P]M] J09:1260e - NNUp +% - .Nu]Po]Ns]P:p]S] J09:1260f - YF +. - . J09:1260g - NN1u Sedimentation sedimentation [S[Np:S. J09:1260h - NN2 coefficients coefficient .Np:S] J09:1260i - VBDR were be [Vwp. J09:1260j - VVNv computed compute .Vwp] J09:1270a - IIa as as [P:e. J09:1270b - FO - [Np. J09:1270c - NN2 values value .Np]P:e] J09:1270d - CC and and [S+. J09:1270e - JJ relative relative [Np:S. J09:1270f - NN2 amounts amount . J09:1270g - IO of of [Po. J09:1270h - AT the the [Np. J09:1270i - JJ various various . J09:1270j - NN2 components component .Np]Po]Np:S] J09:1270k - VBDR were be [Vwp. J09:1270m - VVNv calculated calculate .Vwp] J09:1280a - II from from [P:h. J09:1280b - AT the the [Np. J09:1280c - NP1s Schlieren Schlieren [Nns.Nns] J09:1280d - NN2 patterns pattern .Np]P:h]S+]S] J09:1280e - YF +. - .O] J09:1280f - YB - [Oh.Oh] J09:1280g - IF For for [O[S[P:c. J09:1280h - JJ preparative preparative [Ns. J09:1280i - NN1u ultracentrifugation ultracentrifugation .Ns]P:c] J09:1280j - YC +, - . J09:1290a - NN2 fractions fraction [Np:S.Np:S] J09:1290b - II from from [P:q. J09:1290c - AT the the [Ns. J09:1290d - NN1c column column .Ns]P:q] J09:1290e - VBDR were be [Vwp. J09:1290f - VVNv concentrated concentrate .Vwp] J09:1290g - IIb by by [Pb:a. J09:1290h - JJ negative negative [Ns. J09:1290i - NN1n pressure pressure . J09:1300a - NN1u dialysis dialysis .Ns]Pb:a] J09:1300b - IIt to to [P:e. J09:1300c - NN2 volumes volume [Np. J09:1300d - IO of of [Po. J09:1300e - MC1n 1 - [Nu. J09:1300f S NNU ml - . J09:1300g - CCr or or [D+. J09:1300h - DAR less less .D+]Nu]Po]Np]P:e] J09:1300i - YC +, - . J09:1300j - VVNv transferred transfer [S-[Vn.Vn] J09:1300k - IIt to to [P:q. J09:1300m - NN1u cellulose cellulose [Np. J09:1310a - NN2 tubes tube .Np]P:q]S-] J09:1310b - CC and and [S+. J09:1310c - VVNt diluted dilute [Vn.Vn] J09:1310d - IIt to to [P:e. J09:1310e - MCn 12 - [Nu. J09:1310f S NNU ml - .Nu]P:e] J09:1310g - IW with with [P:h. J09:1310h - JJ isotonic isotonic [Ns. J09:1310i - NN1u saline saline .Ns]P:h]S+]S] J09:1310j - YF +. - . J09:1310k - NN1u Ultracentrifugation ultracentrifugation [S[Ns:S.Ns:S] J09:1310m - YG - - [t163.t163] J09:1320a - VBDZ was be [Vsp. J09:1320b - RTn then then [Rsw:G163.Rsw:G163] J09:1320c - VVNv carried carry .Vsp] J09:1320d - RP out out [R:n.R:n] J09:1320e - II in in [P:p. J09:1320f - AT1 a a [Ns. J09:1320g - NP1j Spinco Spinco [Nns.Nns] J09:1320h - NN1c model model [Nns. J09:1320i S FOs L - .Nns] J09:1320j - NN1c ultracentrifuge ultracentrifuge .Ns]P:p] J09:1320k - II at at [P:h. J09:1330a - MCn 40,000 - [Nu. J09:1330b S NNU rpm - .Nu]P:h] J09:1330c - IF for for [P:t. J09:1330d - MCn 125 - [Nu. J09:1330e - IIt to to [P. J09:1330f - MCn 150 - .P] J09:1330g S NNU min - .Nu]P:t] J09:1330h - YC +, - . J09:1330i - NN1n refrigeration refrigeration [Tg:b[Ns:S.Ns:S] J09:1330j - NN1n being being [Vgp. J09:1330k - VVNt used use .Vgp] J09:1330m - II throughout throughout [P:t. J09:1340a - AT the the [Ns. J09:1340b - NN1c run run .Ns]P:t]Tg:b]S] J09:1340c - YF +. - . J09:1340d - JJ Successive successive [S[Np:S. J09:1340e - MC1n 1 - [Nu. J09:1340f - YH + - . J09:1340g S NNU +ml - .Nu] J09:1340h - NN2 fractions fraction .Np:S] J09:1340i - YG - - [t165.t165] J09:1340j - VBDR were be [Vwp. J09:1340k - RTn then then [Rsw:G165.Rsw:G165] J09:1340m - VVNv drawn draw .Vwp] J09:1340n - RP off off [R:q.R:q] J09:1340p - IW with with [P:h. J09:1340q - AT1 a a [Ns. J09:1350a - JJ hypodermic hypodermic . J09:1350b - NN1c syringe syringe .Ns]P:h] J09:1350c - YC +, - . J09:1350d - VVGv starting start [Tg:b[Vg.Vg] J09:1350e - II at at [P:p. J09:1350f - AT the the [Ns. J09:1350g - NN1c top top . J09:1350h - IO of of [Po. J09:1350i - AT the the [Ns. J09:1350j - NN1n tube tube .Ns]Po]Ns]P:p]Tg:b] J09:1350k - YC +, - . J09:1350m - CC and and [S+. J09:1350n - VVNt tested test [Vn.Vn] J09:1350p - IF for for [P:r. J09:1350q - NN1n agglutinin agglutinin [Ns. J09:1360a - NN1n activity activity .Ns]P:r]S+]S] J09:1360b - YF +. - .O] J09:1360c - YB - [Oh.Oh] J09:1360d - JBo Other other [O[S[Np:S. J09:1360e - NN2 methods method .Np:S] J09:1360f - VMo will will [Vcp. J09:1360g - VB0 be be . J09:1360h - VVNt described describe .Vcp] J09:1360i - RL below below [R:p.R:p]S] J09:1360j - YF +. - .O] J09:1370a - YBL - [Oh. J09:1370b - JJ EXPERIMENTAL experimental [J. J09:1370c - CC AND and [Np+. J09:1370d - NN2 RESULTS result .Np+]J] J09:1370e - YBR - .Oh] J09:1370f - AT The the [O[S[Ns:s167. J09:1370g - JJ insoluble insoluble . J09:1370h - NN1n material material . J09:1370i - DDQr which which [Fr[Dq:s167.Dq:s167] J09:1370j - VVDt precipitated precipitate [Vd.Vd] J09:1380a - II during during [P:t. J09:1380b - NN1u dialysis dialysis .P:t] J09:1380c - II against against [P:h. J09:1380d - VVGv starting start [Ns[Tg[Vg.Vg]Tg] J09:1380e - NN1c buffer buffer .Ns]P:h]Fr]Ns:s167] J09:1380f - RR always always [R:t.R:t] J09:1380g - VVDv showed show [Vd.Vd] J09:1380h - JJ intense intense [Ns:o. J09:1380i - NN1n agglutinin agglutinin . J09:1390a - NN1n activity activity .Ns:o] J09:1390b - YC +, - . J09:1390c - JJ regardless regardless [Jh:c. J09:1390d - IO of of [Po. J09:1390e - AT the the [Ns. J09:1390f - NN1n blood blood . J09:1390g - NNJ1c group group . J09:1390h - IO of of [Po. J09:1390i - AT the the [Ns. J09:1390j - NN1c donor donor .Ns]Po]Ns]Po]Jh:c]S] J09:1390k - YF +. - . J09:1390m - IW With with [S[P:r. J09:1390n - DD1e either either [Ds. J09:1400a - IO of of [Po. J09:1400b - AT the the [Np:169. J09:1400c - NN2 gradients gradient . J09:1400d - YG - - [Tn[S169.S169] J09:1400e - VVNt described describe [Vn.Vn]Tn]Np:169]Po]Ds]P:r] J09:1400f - YC +, - . J09:1400g - NN1u chromatography chromatography [Ns:s. J09:1400h - II on on [P. J09:1400i S FOc DEAE - [Ns. J09:1400j - YH + - . J09:1400k - NN1u +cellulose cellulose .Ns]P]Ns:s] J09:1410a - VVDv separated separate [Vd.Vd] J09:1410b - NN2 agglutinins agglutinins [Np:o. J09:1410c - IO of of [Po. J09:1410d - AT the the [N. J09:1410e S FOs ABO - . J09:1410f - NNc series series .N]Po]Np:o] J09:1410g - II into into [P:q. J09:1410h - RR21 at at [Np[RR=. J09:1410i - RR22 least least .RR=] J09:1410j - MC three three . J09:1410k - NN2 regions region . J09:1420a - YPL ( - . J09:1420b A NNmm +Figs. - [Iu[Nnp. J09:1420c - MC1n 1 - [MC1n&. J09:1420d - CC and and [MCn+. J09:1420e - MCn 2 - .MCn+]MC1n&]Nnp]Iu] J09:1420f - YPR +) - . J09:1420g - YN +: - . J09:1420h - MC1 one one [Ms@. J09:1420i - IO of of [Po. J09:1420j - RR extremely extremely [Ns[J. J09:1420k - JJ low low .J] J09:1420m - JJ anionic anionic . J09:1420n - VVGv binding bind [Tg[Vg.Vg]Tg] J09:1420p - NN1n capacity capacity .Ns]Po] J09:1420q - YC +, - . J09:1430a - MC1 one one [Ms-. J09:1430b - IO of of [Po. J09:1430c - JJ low low [Ns. J09:1430d - JJ anionic anionic . J09:1430e - VVGv binding bind [Tg[Vg.Vg]Tg] J09:1430f - NN1n capacity capacity .Ns]Po]Ms-] J09:1430g - CC and and [Ms+. J09:1430h - MC1 one one . J09:1430i - IO of of [Po. J09:1430j - JJ high high [Ns. J09:1430k - JJ anionic anionic . J09:1430m - VVGv binding bind [Tg[Vg.Vg]Tg] J09:1440a - NN1n capacity capacity .Ns]Po]Ms+]Ms@]Np]P:q]S] J09:1440b - YF +. - . J09:1440c - DD2i These these [S[Dp:S.Dp:S] J09:1440d - VH0 have have [Vfp. J09:1440e - VBN been be . J09:1440f - VVNt labeled label .Vfp] J09:1440g - NN2 Regions region [Nnp:e. J09:1440h - MC1n 1 - [Ms. J09:1440i - YC +, - . J09:1440j - MCn 2 - [M-.M-] J09:1440k - YC +, - . J09:1440m - CC and and [M+. J09:1440n - MCn 4 - .M+] J09:1440p - YC +, - . J09:1440q - RAc respectively respectively . J09:1440r - YC +, - .Ms]Nnp:e] J09:1450a - II in in [P:p. J09:1450b A NNm Fig. - [Nns. J09:1450c - MC1n 1 - .Nns]P:p]S] J09:1450d - YF +. - . J09:1450e - CSn When when [S[Fa:t[Rq:t.Rq:t] J09:1450f - AT the the [Ns:S. J09:1450g - JJ early early . J09:1450h - NN1c part part . J09:1450i - IO of of [Po. J09:1450j - AT the the [Ns. J09:1450k - NN1c gradient gradient .Ns]Po]Ns:S] J09:1450m - VBDZ was be [Vsp. J09:1450n - VVNv flattened flatten .Vsp] J09:1450p - YC +, - . J09:1460a - LEe either either [Pb:h. J09:1460b - IIb by by . J09:1460c - VVGt using use [Tg[Vg.Vg] J09:1460d - AT the the [Ns:o171. J09:1460e - NN1c gradient gradient . J09:1460f - YG - - [Tn[S171.S171] J09:1460g - VVNv shown show [Vn.Vn] J09:1460h - II in in [P:p. J09:1460i A NNm Fig. - [Nns. J09:1460j - MCn 2 - .Nns]P:p]Tn]Ns:o171]Tg] J09:1460k - CCr or or [Pb+. J09:1460m - IIb by by . J09:1460n - VVGv allowing allow [Tg[Vg.Vg] J09:1460p - AT the the [Ns:O173. J09:1460q - YIL - . J09:1460r - NN1c +cone cone [N. J09:1460s - YH + - . J09:1460t - NN1c +sphere sphere .N] J09:1460u - YIR + - . J09:1470a - NN1c gradient gradient .Ns:O173] J09:1470b - YG - - [Ti:o[s173.s173] J09:1470c - TO to to [Vi. J09:1470d - VV0v become become .Vi] J09:1470e - VVNt established establish [Tn:e[Vn.Vn]Tn:e] J09:1470f - DAR more more [R:h. J09:1470g - RR slowly slowly .R:h]Ti:o]Tg]Pb+]Pb:h]Fa:t] J09:1470h - YC +, - . J09:1470i - NN1c Region region [Ns:S[Nns. J09:1470j - MCn 2 - .Nns] J09:1470k - NN1n activity activity .Ns:S] J09:1470m - YG - - [t175.t175] J09:1480a - VMd could can [Vdcp. J09:1480b - RR sometimes sometimes [R:G175.R:G175] J09:1480c - VB0 be be . J09:1480d - VVNv separated separate .Vdcp] J09:1480e - II into into [P:q. J09:1480f - MC two two [Np. J09:1480g - NN2 areas area . J09:1480h - YPL ( - . J09:1480i - NN2 +donors donor [Iu[Nn[Nn. J09:1480j A NP1i P. - . J09:1480k A NP1i J. - . J09:1490a - CC and and [Nns+. J09:1490b A NP1i R. - . J09:1490c A NP1i S. - .Nns+] J09:1490d - YC +, - . J09:1490e A NNm Fig. - [Nns@. J09:1490f - MC1n 1 - .Nns@]Nn] J09:1490g - CC and and [Nns+. J09:1490h A NP1i E. - . J09:1490i A NP1i M. - . J09:1490j - YC +, - . J09:1490k A NNm Fig. - [Nns@. J09:1490m - MCn 2 - .Nns@]Nns+]Nn]Iu] J09:1490n - YPR +) - .Np]P:q]S] J09:1490p - YF +. - . J09:1490q - AT The the [S[Ns:s. J09:1490r - DAr latter latter . J09:1500a - NN1n procedure procedure .Ns:s] J09:1500b - VVDv gave give [Vd.Vd] J09:1500c - NNL1c rise rise [Ns:o.Ns:o] J09:1500d - IIt to to [P:u. J09:1500e - AT1 a a [Ns. J09:1500f - JJ small small . J09:1500g - JJ active active [Ns. J09:1500h - NN1n protein protein .Ns] J09:1500i - NN1c peak peak .Ns]P:u] J09:1500j - YPL ( - . J09:1500k - NN1c +Region region [Iu[Nns. J09:1500m - MCb 1a - .Nns]Iu] J09:1500n - YPR +) - . J09:1500p - II between between [P:p. J09:1510a - NN2 Regions region [Nnp. J09:1510b - MC1n 1 - [MC1n&. J09:1510c - CC and and [MCn+. J09:1510d - MCn 2 - .MCn+]MC1n&]Nnp]P:p]S] J09:1510e - YF +. - . J09:1510f - II In in [S[P:p. J09:1510g - MCn 2 - [Np[M. J09:1510h - IO of of [Po. J09:1510i - MCn 15 - .Po]M] J09:1510j - NN2 experiments experiment . J09:1510k - II on on [P. J09:1510m - JJ whole whole [Ns. J09:1510n - NN1u serum serum .Ns]P]Np]P:p] J09:1510p - AT1 a a [Ns:S. J09:1510q - NN1c region region . J09:1510r - IO of of [Po. J09:1520a - NN1n agglutinin agglutinin [Ns. J09:1520b - NN1n activity activity . J09:1520c - IW with with [P. J09:1520d - JJ intermediate intermediate [Ns. J09:1520e - JJ anionic anionic . J09:1520f - VVGv binding bind [Tg[Vg.Vg]Tg] J09:1520g - NN1n capacity capacity .Ns]P]Ns]Po]Ns:S] J09:1520h - VBDZ was be [Vsp. J09:1520i - VVNt detected detect .Vsp] J09:1530a - YPL ( - . J09:1530b - NN1c +Region region [Nns@. J09:1530c - MCn 3 - . J09:1530d - YC +, - . J09:1530e A NNm Fig. - [Nns@. J09:1530f - MC1n 1 - .Nns@]Nns@] J09:1530g - YPR +) - .S] J09:1530h - YF +. - . J09:1530i - RR Moreover moreover [S[R:m.R:m] J09:1530j - YC +, - . J09:1530k - ICSt after after [P:t. J09:1530m - NN1n concentration concentration .P:t] J09:1530n - VVGt using use [Tg:h[Vg.Vg] J09:1530p - JJ negative negative [Ns:o[Ns. J09:1540a - NN1n pressure pressure .Ns] J09:1540b - NN1u dialysis dialysis .Ns:o]Tg:h] J09:1540c - YC +, - . J09:1540d - NN1n agglutinin agglutinin [Ns:S. J09:1540e - NN1n activity activity .Ns:S] J09:1540f - YG - - [t177.t177] J09:1540g - VMd could can [Vdcp. J09:1540h - RR sometimes sometimes [R:G177.R:G177] J09:1540i - VB0 be be . J09:1540j - VVNt detected detect .Vdcp] J09:1550a - II in in [P:p. J09:1550b - AT the the [Ns:179. J09:1550c - NN1c region region . J09:1550d - YG - - [Tn[S179.S179] J09:1550e - VVNt designated designate [Vn.Vn] J09:1550f - MCb 2a - [M:e.M:e]Tn] J09:1550g - YPL ( - . J09:1550h - NN2 +donors donor [Iu[Nn. J09:1550i A NP1i P. - . J09:1550j A NP1i J. - . J09:1550k - YC +, - . J09:1550m A NP1i D. - [Nns-. J09:1550n A NP1i A. - .Nns-] J09:1550p - YC +, - . J09:1550q - CC and and [Nns+. J09:1560a A NP1i J. - . J09:1560b A NP1i F. - .Nns+] J09:1560c - YC +, - . J09:1560d A NNm Fig. - [Nns@. J09:1560e - MC1n 1 - .Nns@]Nn]Iu] J09:1560f - YPR +) - .Ns:179]P:p]S] J09:1560g - YF +. - .O] J09:1560h - YB - [Oh.Oh] J09:1560i - XX Not not [O[S. J09:1560j - DBa all all [Np:s. J09:1560k - DD2i these these . J09:1560m - NN2 regions region .Np:s] J09:1560n - VVDt exhibited exhibit [Vd.Vd] J09:1560p - JJ equal equal [Ns:o. J09:1570a - VVGt agglutinating agglutinate [Tg[Vg.Vg]Tg] J09:1570b - NN1n activity activity .Ns:o] J09:1570c - YC +, - . J09:1570d - CSA as as [A:x. J09:1570e - VVNt evidenced evidence [Vn.Vn] J09:1570f - IIb by by [Pb:a. J09:1570g - NN1n titer titre [N. J09:1570h - CC and and [Ns+. J09:1570i - AT the the . J09:1570j - NN1u extent extent . J09:1570k - IO of of [Po. J09:1570m - AT the the [Np. J09:1580a - JJ active active . J09:1580b - NN2 areas area .Np]Po]Ns+]N]Pb:a]A:x]S] J09:1580c - YF +. - . J09:1580d - II In in [S[P:m. J09:1580e - DBa all all [Np. J09:1580f - NN2 cases case .Np]P:m] J09:1580g - YC +, - . J09:1580h - DAT most most [Ds:s. J09:1580i - IO of of [Po. J09:1580j - AT the the [Ns. J09:1580k - NN1n activity activity .Ns]Po]Ds:s] J09:1580m - VVDi lay lie [Vd.Vd] J09:1580n - II in in [P:p. J09:1580p - AT the the [Ns. J09:1580q - NN1c region region . J09:1580r - IO of of [Po. J09:1590a - JJ high high [Ns. J09:1590b - JJ anionic anionic . J09:1590c - VVGv binding bind [Tg[Vg.Vg]Tg] J09:1590d - NN1n capacity capacity .Ns]Po]Ns]P:p]S] J09:1590e - YF +. - . J09:1590f - DD1i This this [S[Ds:s.Ds:s] J09:1590g - VBDZ was be [Vsb.Vsb] J09:1590h - RR particularly particularly [J:e. J09:1590i - JJ noticeable noticeable .J:e] J09:1590j - II in in [P:p. J09:1590k - NNJ1c group group [Np:181[Nn. J09:1600a S ZZ1 A - . J09:1600b - CC and and [Nns+. J09:1600c - NNJ1c group group . J09:1600d S ZZ1 B - .Nns+]Nn] J09:1600e - NN2 sera serum . J09:1600f - YC +, - . J09:1600g - II in in [Fr[Pq:m. J09:1600h - DDQr which which [Nqp:181. J09:1600i - NN2 cases case .Nqp:181]Pq:m] J09:1600j - NN1n activity activity [Ns:s. J09:1600k - II in in [P. J09:1600m - NN2 Regions region [Nnp. J09:1600n - MC1n 1 - [MC1n&. J09:1610a - CC and and [MCn+. J09:1610b - MCn 2 - .MCn+]MC1n&]Nnp]P]Ns:s] J09:1610c - VBDZ was be [Vsb.Vsb] J09:1610d - RR usually usually [R:m.R:m] J09:1610e - XX not not . J09:1610f - JJ detectable detectable [J:e.J:e] J09:1610g - IW without without [P:h. J09:1610h - JJ prior prior [Ns. J09:1610i - NN1n concentration concentration .Ns]P:h] J09:1610j - CC and and [Fr+. J09:1610k - RR occasionally occasionally [R:t.R:t] J09:1620a - VMd could can [Vdcep. J09:1620b - XX not not . J09:1620c - VB0 be be . J09:1620d - VVNt detected detect .Vdcep] J09:1620e - RR21 at at [R:m[RR=. J09:1620f - RR22 all all .RR=]R:m]Fr+]Fr]Np:181]P:p]S] J09:1620g - YF +. - . J09:1620h - EX There there [S. J09:1620i - VVDi appeared appear [Vd.Vd] J09:1620j - TO to to [Ti:s[Vib. J09:1620k - VB0 be be .Vib] J09:1620m - ATn no no [Ns:s. J09:1620n - NN1n difference difference . J09:1630a - II in in [P. J09:1630b - AT the the [Ns. J09:1630c - NN1n distribution distribution . J09:1630d - IO of of [Po. J09:1630e - FB anti anti [Ns[J. J09:1630f - YH + - . J09:1630g S ZZ1 +A - . J09:1630h - CC and and [J+. J09:1630i - FB anti anti . J09:1630j - YH + - . J09:1630k S ZZ1 +B - .J+]J] J09:1630m - NN1n activity activity .Ns]Po]Ns]P]Ns:s] J09:1630n - II in in [P:p. J09:1630p - NNJ1c group group [Ns[Nns. J09:1640a S ZZ1 O - .Nns] J09:1640b - NN1u serum serum .Ns]P:p]Ti:s] J09:1640c - YC +, - . J09:1640d - CSg though though [Fa:c. J09:1640e - II in in [P:p. J09:1640f - MC two two [Np. J09:1640g - NNJ1c group group [Nns. J09:1640h S ZZ1 O - .Nns] J09:1640i - NN2 donors donor . J09:1640j - YPL ( - . J09:1640k A NP1i +J. - [Nn@. J09:1640m A NP1i F. - . J09:1640n - CC and and [Nns+. J09:1640p A NP1i E. - . J09:1650a A NP1i M. - .Nns+]Nn@] J09:1650b - YPR +) - .Np]P:p] J09:1650c - RRx only only [R:m.R:m] J09:1650d - MC1 one one [Ns:S. J09:1650e - NN1n type type . J09:1650f - IO of of [Po. J09:1650g - NN1n agglutinin agglutinin .Po]Ns:S] J09:1650h - VBDZ was be [Vsp. J09:1650i - VVNt found find .Vsp] J09:1650j - II in in [P:p. J09:1650k - AT the the [Np. J09:1650m - NN2 regions region . J09:1650n - IO of of [Po. J09:1650p - JJ low low [Ns. J09:1650q - JJ anionic anionic . J09:1660a - VVGv binding bind [Tg[Vg.Vg]Tg] J09:1660b - NN1n capacity capacity .Ns]Po]Np]P:p] J09:1660c - YPL ( - . J09:1660d A NNmm +Figs. - [Iu[Nnp. J09:1660e - MC1n 1 - [MC1n&. J09:1660f - CC and and [MCn+. J09:1660g - MCn 2 - .MCn+]MC1n&]Nnp]Iu] J09:1660h - YPR +) - .Fa:c]S] J09:1660i - YF +. - .O] J09:1660j - YB - [Oh.Oh] J09:1660k - DA2q Several several [O[S[Np:S. J09:1660m - NN2 samples sample . J09:1660n - IO of of [Po. J09:1670a - VVNt citrated citrate [Ns[Tn[Vn.Vn]Tn] J09:1670b - NN1u plasma plasma .Ns]Po]Np:S] J09:1670c - VBDR were be [Vwp. J09:1670d - VVNt fractionated fractionate .Vwp] J09:1670e - II in in [P:p. J09:1670f - APPGi2 our our [Ns. J09:1670g - NN1c laboratory laboratory .Ns]P:p] J09:1670h - IIb by by [Pb:h. J09:1670i - NN1n Method method [Nns. J09:1670j - MCn 6 - . J09:1670k - IO of of [Po. J09:1670m - NP1s Cohn Cohn [Nns. J09:1680a - YTL - . J09:1680b - RAc21 et et [RAc=. J09:1680c - RAc22 al al .RAc=] J09:1680d - YTR - .Nns]Po]Nns]Pb:h]S] J09:1680e - YF +. - . J09:1680f - DD2i These these [S[Np:S. J09:1680g - NN2 fractions fraction .Np:S] J09:1680h - VBDR were be [Vwp. J09:1680i - VVNt tested test .Vwp] J09:1680j - IF for for [P:r. J09:1680k S FOs ABO - [Ns. J09:1680m - NN1n agglutinin agglutinin . J09:1690a - NN1n activity activity .Ns]P:r] J09:1690b - YC +, - . J09:1690c - VVGt using use [Tg:b[Vg.Vg] J09:1690d - NN2 fractions fraction [Np:o. J09:1690e - II from from [P. J09:1690f - NNJ1c group group [Ns[Nns. J09:1690g S FOs AB - .Nns] J09:1690h - NN1u plasma plasma .Ns]P]Np:o] J09:1690i - IIa as as [P:j. J09:1690j - AT1 a a [Ns. J09:1690k - NN1n control control .Ns]P:j]Tg:b]S] J09:1690m - YF +. - . J09:1700a - CSA As as [S[A:x. J09:1700b - VVNt expected expect [Vn.Vn]A:x] J09:1700c - YC +, - . J09:1700d - DAT most most [Ds:S. J09:1700e - IO of of [Po. J09:1700f - AT the the [Ns. J09:1700g - NN1n activity activity .Ns]Po]Ds:S] J09:1700h - VBDZ was be [Vsp. J09:1700i - VVNt found find .Vsp] J09:1700j - II in in [P:p. J09:1700k - NN1c Fraction fraction [Nns. J09:1700m - FO - .Nns]P:p] J09:1700n - YC +, - . J09:1700p - IW with with [W:b. J09:1710a - JJ slight slight [Ns:S. J09:1710b - NN1n activity activity .Ns:S] J09:1710c - VVNv seen see [Vn.Vn] J09:1710d - II in in [P:p. J09:1710e - NN1c Fraction fraction [Nns. J09:1710f - MCr IV - . J09:1710g - YH + - . J09:1710h - MC1n +1 - .Nns]P:p]W:b]S] J09:1710i - YF +. - . J09:1710j - AT1 A a [S[Ns:S. J09:1710k - NN1c sample sample . J09:1710m - IO of of [Po. J09:1710n - NN1c Fraction fraction [Nns. J09:1710p - FO - .Nns]Po] J09:1720a - II from from [P. J09:1720b - NNJ1c group group [Ns[Nns. J09:1720c S ZZ1 O - .Nns] J09:1720d - NN1u plasma plasma .Ns]P]Ns:S] J09:1720e - VBDZ was be [Vsp. J09:1720f - VVNv dissolved dissolve .Vsp] J09:1720g - II in in [P:p. J09:1720h - VVGv starting start [Ns[Tg[Vg.Vg]Tg] J09:1720i - NN1c buffer buffer .Ns]P:p] J09:1720j - YC +, - . J09:1720k - VVNt dialyzed dialyse [S-[Vn.Vn] J09:1720m - II against against [P:h. J09:1730a - DD1i this this [Ns. J09:1730b - NN1c buffer buffer .Ns]P:h]S-] J09:1730c - CC and and [S+. J09:1730d - VVNt subjected subject [Vn.Vn] J09:1730e - IIt to to [P:u. J09:1730f - NN1u chromatography chromatography .P:u] J09:1730g - VVGt using use [Tg:h[Vg.Vg] J09:1730h - AT the the [Ns:o183. J09:1730i - NN1c gradient gradient . J09:1730j - YG - - [Tn[S183.S183] J09:1730k - VVNv shown show [Vn.Vn] J09:1740a - II in in [P:p. J09:1740b A NNm Fig. - [Nns. J09:1740c - MCn 2 - .Nns]P:p]Tn]Ns:o183]Tg:h]S+]S] J09:1740d - YF +. - . J09:1740e - RR21 Once once [S[R:t[RR=. J09:1740f - RR22 again again .RR=]R:t] J09:1740g - YC +, - . J09:1740h - LE both both [Np:S[J. J09:1740i - FB anti anti . J09:1740j - YH + - . J09:1740k S ZZ1 +A - . J09:1740m - CC and and [J+. J09:1740n - FB anti anti . J09:1740p - YH + - . J09:1740q S ZZ1 +B - .J+]J] J09:1740r - NN2 activities activity .Np:S] J09:1750a - VBDR were be [Vwp. J09:1750b - VVNt found find .Vwp] J09:1750c - II in in [P:p. J09:1750d - AT the the [Ns:185. J09:1750e - JJ insoluble insoluble . J09:1750f - NN1n material material . J09:1750g - YG - - [Tn[S185.S185] J09:1750h - VVNt precipitated precipitate [Vn.Vn] J09:1750i - II during during [P:t. J09:1750j - NN1u dialysis dialysis .P:t]Tn]Ns:185]P:p]S] J09:1750k - YF +. - . J09:1760a - RR Similarly similarly [S[R:h.R:h] J09:1760b - YC +, - . J09:1760c - DB2 both both [Np:S. J09:1760d - NN2 types type . J09:1760e - IO of of [Po. J09:1760f - NN2 antibodies antibody .Po]Np:S] J09:1760g - VBDR were be [Vwp. J09:1760h - VVNt found find .Vwp] J09:1760i - II in in [P:p. J09:1760j - MC three three [Np:187. J09:1760k - NN2 regions region . J09:1760m - IO of of [Po. J09:1760n - AT the the [Ns. J09:1770a - JJ chromatographic chromatographic . J09:1770b - NN1n eluate eluate .Ns]Po] J09:1770c - YC +, - . J09:1770d - YG - - [Tg[s187.s187] J09:1770e - VHG having have [Vg.Vg] J09:1770f - RR extremely extremely [Ns:o[J. J09:1770g - JJ low low . J09:1770h - YC +, - . J09:1770i - JJ low low [J-.J-] J09:1770j - YC +, - . J09:1770k - CC and and [J+. J09:1770m - JJ high high .J+]J] J09:1770n - JJ anionic anionic . J09:1780a - VVGv binding bind [Tg[Vg.Vg]Tg] J09:1780b - NN1n capacity capacity .Ns:o] J09:1780c - YC +, - . J09:1780d - RAc respectively respectively [R:m.R:m] J09:1780e - YPL ( - . J09:1780f A NNm +Fig. - [Iu[Nns. J09:1780g - MCn 3 - .Nns]Iu] J09:1780h - YPR +) - .Tg]Np:187]P:p]S] J09:1780i - YF +. - .O] J09:1780j - YB - [Oh.Oh] J09:1780k - NN1u Chromatography chromatography [O[S[Ns:s. J09:1780m - IO of of [Po. J09:1790a - JJ whole whole [Np. J09:1790b - NN2 sera serum .Np]Po]Ns:s] J09:1790c - VVDt revealed reveal [Vd.Vd] J09:1790d - CST that that [Fn:o. J09:1790e - AT the the [Np:s. J09:1790f - NN2 areas area . J09:1790g - IO of of [Po. J09:1790h S FWs Rh - [Ns[Nn.Nn] J09:1790i - NN1c antibody antibody . J09:1790j - NN1n activity activity .Ns]Po]Np:s] J09:1790k - VBDR were be [Vwb.Vwb] J09:1790m - RR generally generally [J:e. J09:1800a - JJ continuous continuous [JJ&. J09:1800b - CC and and [JJ+. J09:1800c - JJ wide wide .JJ+]JJ&]J:e]Fn:o]S] J09:1800d - YF +. - . J09:1800e - AT The the [S[Ns:s. J09:1800f - JJ incomplete incomplete . J09:1800g - NN1c antibody antibody . J09:1800h - NN1n activity activity .Ns:s] J09:1800i - VVDi appeared appear [Vd.Vd] J09:1810a - II in in [P:p. J09:1810b - AT the the [Ns. J09:1810c - JJ early early . J09:1810d - NN1c part part . J09:1810e - IO of of [Po. J09:1810f - AT the the [Ns. J09:1810g - NN1c chromatogram chromatogram .Ns]Po]Ns]P:p] J09:1810h - YS +; - . J09:1810i - AT the the [S-[Nj:s. J09:1810j - JJ complete complete .Nj:s] J09:1810k - YC +, - . J09:1810m - II in in [P:p. J09:1810n - AT the the [Ns. J09:1810p - DAr latter latter . J09:1820a - NN1c part part .Ns]P:p]S-]S] J09:1820b - YF +. - . J09:1820c - AT The the [S[Ns:s189. J09:1820d - NN1u serum serum . J09:1820e - YG - - [Tg[s189.s189] J09:1820f - VVGt containing contain [Vg.Vg] J09:1820g - AT the the [Ns:o. J09:1820h - JJ mixed mixed . J09:1820i - NN1n type type . J09:1820j - IO of of [Po. J09:1820k - JJ complete complete [Np[JJ&. J09:1820m - CC and and [JJ+. J09:1820n - JJ incomplete incomplete .JJ+]JJ&] J09:1830a - NN2 antibodies antibody .Np]Po]Ns:o]Tg]Ns:s189] J09:1830b - VVDv showed show [Vd.Vd] J09:1830c - NN1n activity activity [Ns:o.Ns:o] J09:1830d - II in in [P:p. J09:1830e - DB2 both both [Np. J09:1830f - NN2 regions region .Np]P:p] J09:1830g - YPL ( - . J09:1830h A NNm +Fig. - [Iu[Nns. J09:1830i - MC1n 1 - .Nns]Iu] J09:1830j - YPR +) - .S] J09:1830k - YF +. - . J09:1830m - II In in [S[P:m. J09:1830n - DBa all all [Np. J09:1830p - NN2 cases case .Np]P:m] J09:1840a - AT the the [Ns:S. J09:1840b - NN1n activity activity . J09:1840c - II against against [P. J09:1840d - FO - [Np. J09:1840e - NN2 cells cell .Np]P]Ns:S] J09:1840f - VBDZ was be [Vsp. J09:1840g - VVNv spread spread .Vsp] J09:1840h - II over over [P:p. J09:1840i - AT1 a a [Ns. J09:1840j - JJR wider wide [Jh. J09:1840k - YG - - [190.190]Jh] J09:1840m - NN1n area area . J09:1840n - CSN than than [P:190. J09:1840p - DD1a that that [Ds. J09:1850a - IW with with [P. J09:1850b - FO - [Np. J09:1850c - NN2 cells cell .Np]P]Ds]P:190]Ns]P:p] J09:1850d - YC +, - . J09:1850e - JJ regardless regardless [Jh:c. J09:1850f - IO of of [Po. J09:1850g - AT the the [Ns:191. J09:1850h - NN1n type type . J09:1850i - IO of of [Po. J09:1850j - NN1c test test .Po] J09:1850k - YPL ( - . J09:1850m - NN1u +saline saline [N@. J09:1850n - YC +, - . J09:1850p - NN1u albumin albumin [Ns-.Ns-] J09:1850q - YC +, - . J09:1850r - JJ indirect indirect [Nns-. J09:1860a - NP1s Coombs Coombs .Nns-]N@] J09:1860b - YPR +) - . J09:1860c - YG - - [Tn[S191.S191] J09:1860d - VVNt used use [Vn.Vn] J09:1860e - IF for for [P:c. J09:1860f - NN1n comparison comparison .P:c]Tn]Ns:191]Po]Jh:c]S] J09:1860g - YF +. - . J09:1860h - AT The the [S[Ns:s193. J09:1860i - JJ insoluble insoluble . J09:1860j - NN1n material material . J09:1860k - YG - - [Tg[s193.s193] J09:1860m - VVGi resulting result [Vg.Vg] J09:1870a - II from from [P:q. J09:1870b - NN1u dialysis dialysis [Ns. J09:1870c - II against against [P. J09:1870d - VVGv starting start [Ns[Tg[Vg.Vg]Tg] J09:1870e - NN1c buffer buffer .Ns]P]Ns]P:q]Tg]Ns:s193] J09:1870f - RR always always [R:t.R:t] J09:1870g - VVDv showed show [Vd.Vd] J09:1870h - JJ strong strong [Ns:o. J09:1870i - NN1n activity activity .Ns:o]S] J09:1870j - YF +. - . J09:1870k - RR21 In in [S[R:m[RR=. J09:1880a - RR22 fact fact .RR=]R:m] J09:1880b - NN1u agglutination agglutination [Ns:S. J09:1880c - IO of of [Po. J09:1880d - FO - [Np. J09:1880e - NN2 cells cell .Np]Po] J09:1880f - II in in [P. J09:1880g - NN1u saline saline .P]Ns:S] J09:1880h - VMd could can [Vdcp. J09:1880i - VB0 be be . J09:1880j - VVNv produced produce .Vdcp] J09:1880k - IIb by by [Pb:h. J09:1880m - AT the the [Ns:195. J09:1890a - JJ insoluble insoluble . J09:1890b - NN1n material material . J09:1890c - II from from [P. J09:1890d - NN2 sera serum .P] J09:1890e - YG - - [Tg[s195.s195] J09:1890f - VVGt containing contain [Vg.Vg] J09:1890g - YIL - . J09:1890h - RRx +only only [R:m.R:m] J09:1890i - YIR + - . J09:1890j - JJ incomplete incomplete [Ns:o. J09:1890k - NN1c antibody antibody . J09:1900a - NN1n activity activity .Ns:o]Tg]Ns:195]Pb:h]S] J09:1900b - YF +. - . J09:1900c - DD1i This this [S[Ds:S197.Ds:S197] J09:1900d - YG - - [t199.t199] J09:1900e - VBDZ was be [Vsp. J09:1900f - RRR later late [R:G199.R:G199] J09:1900g - VVNv known know .Vsp] J09:1900h - YG - - [Ti:o[s197.s197] J09:1900i - TO to to [Vib. J09:1900j - VB0 be be .Vib] J09:1900k - AT the the [Ns:e. J09:1900m - NN1n result result . J09:1900n - IO of of [Po. J09:1900p - VVGv concentrating concentrate [Tg[Vg.Vg] J09:1900q - AT the the [Ns:o201. J09:1910a - JJ minute minute . J09:1910b - NN1c amount amount . J09:1910c - IO of of [Po. J09:1910d - JJ complete complete [Ns. J09:1910e - NN1c antibody antibody .Ns]Po] J09:1910f - YG - - [Tn[S201.S201] J09:1910g - VVNt found find [Vn.Vn] J09:1910h - II in in [P:p. J09:1910i - DD2i these these [Np. J09:1910j - NN2 sera serum .Np]P:p]Tn]Ns:o201]Tg]Po]Ns:e]Ti:o] J09:1910k - YS +; - . J09:1910m - CSn when when [S-[Fa:t[Rq:t.Rq:t] J09:1910n - AT the the [Ns:S. J09:1910p - JJ insoluble insoluble . J09:1920a - NN1c fraction fraction .Ns:S] J09:1920b - VBDZ was be [Vsp. J09:1920c - VVNt suspended suspend .Vsp] J09:1920d - II in in [P:p. J09:1920e - AT1 a a [Ns. J09:1920f - NN1n volume volume . J09:1920g - IO of of [Po. J09:1920h - NN1u saline saline [Ns.Ns]Po] J09:1920i - JJ equal equal [Jh. J09:1920j - IIt to to [P. J09:1920k - DD1a that that [Ds. J09:1920m - IO of of [Po. J09:1930a - AT the the [Ns. J09:1930b - JJ original original . J09:1930c - NN1u serum serum . J09:1930d - NN1c sample sample .Ns]Po]Ds]P]Jh]Ns]P:p]Fa:t] J09:1930e - YC +, - . J09:1930f - ATn no no [Ns:S. J09:1930g - JJ complete complete . J09:1930h - NN1c antibody antibody . J09:1930i - NN1n activity activity .Ns:S] J09:1930j - VMd could can [Vdcp. J09:1930k - VB0 be be . J09:1930m - VVNt detected detect .Vdcp]S-]S] J09:1930n - YF +. - .O]